BamHI相关论文
The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp......
The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lin
Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4 expression ......
在对约万名孕妇进行人绒毛生长催乳激素(hCS)检测时,发现两个hCS完全缺乏的病例。她们都有过一次正常妊娠史,体检正常。分娩后,从......
胰岛素依赖性糖尿病(IDDM)和特定的HLA型之间的关系已为人们所认识.HLA抗原的基因位于第6号染色体短臂上,存在于A、B、C、D(DR、D......
Objectives To construct a recombinant plasmid carrying enhanced green fluore- scent protein (EGFP) and human vascular en......
原核表达幽门螺杆菌(Helicobacter pylori,H.pylori)酸适应感应蛋白CrdS,探讨其酸适应的调控机制。提取H.pylori26695标准株全基因......
目的:构建同时携带低氧诱导因子-lα(HIF-lα)和角质细胞生长因子(KGF)的腺病毒载体(pAdxsi-GFP-HIF-KGF),观察其在防治肺损伤潜在......
Cloning and RNAi Construction of a LEAFY Homologous Gene from Populus tomentosa and Preliminary Stud
PtLFY, a LEAFY (LFY) gene, was cloned from Populus tomentosa (LM50) by PCR. Sequencing analysis indicated that PtLFY was......
The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima (Vahl.) Fern was constructed ......
丙型肝炎病毒(HCV)E2糖蛋白能与人细胞的CD81分子结合,CD81分子被视为HCV的受体,并且CD81同E2的相互作用与HCV感染有关。为探讨HC......
本文应用化学法测定了克隆的adr亚型HBV(_pADR-1)DNA全顺序,共长3215个碱基对(bp),比Ono等报道的adr_pHBr330长27个碱基对。_pADR-......
AIM:To compare the efficiency of different plasmids asDNA vectors by cloning three HBsAg-encoded genes intotwo eukaryot......
The essential characteristic of DNA computation is its massive parallelism in obtaining and managing information. With t......
AIM:In recent years,studies have suggested that Epstein-Barr virus(EBV)is associated with HCC.The present studywas to d......
目的旨在构建一种新型的表面蛋白防龋疫苗—DNA防龋疫苗。方法从表面蛋白pac基因上利用BamHI和Sacl双酶切，获得一个3281bp的片段。......
目的:构建重组质粒pPHU281-C-Spec-E,用于敲除菌毛蛋白次要组分基因fimCDE。构建fimCDE缺陷的牙龈卟啉单胞菌,为进一步研究FimCDE......
目的获得中国人的肺表面活性物质结合蛋白A（SP-A） 6A cDNA基因克隆，并进行序列分析，为进一步研究中国人SP-A基因型特点提供方法和资料，......
Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoni
AIM:To construct pEGFP-hepatocyte growth factor(HGF)expression vector,the to detect its expression in transfectedhuman ......
AIM:To evaluate the effects of animal milk containingfucosylated antigens on Helicobacter pylod (Hpylon) bindingto Lewi......
The transfection of NIH 3T3 cells was performed with DNAs from 2 duck primary hepatic carcinomas (DHC 40K, 9K) and I tu......
Two coding sequences for storage protein in Glycine max have been cloned. They were characterized by molecular hybridiz......
Human mitochondrial DNA (mtDNA) restriction endonuclease fragment patterns were analyzed using placenta DNA isolated fr......
CONSTRUCTION OF Sesbania rostrata COSMID GENOMIC LIBRARY AND MOLECULAR CLONING OF LEGHEMOGLOBIN GENE
Using plant mini-Ti cosmid pEND4K, the cosmid genomic library of Sesbania rostratawas constructed. Sizes of plant DNA in......
MOLECULAR CLONING AND NUCLEOTIDE SEQUENCE ANALYSIS OF A TRANSFORMING GENE FROM HUMAN GASTROCARCINOMA
Mouse and rat fibroblasts were transfected with total DNA from human gastrocarcinoma cell line BGC-823. It was shown by......
We have cloned the HBV genome subtype adr through its HindⅢ site into plasmid pBR.322. Twelve recombinant plasmids eac......
Using a system to study promoter activity, we have obtained a promoter fragment from E. coli chromosomal DNA. The HBsAg......
STUDY ON THE GENOME OF CAULIFLOWER MOSAIC VIRUS (XINJIANG ISOLATE)——RESTRICTION MAPPING OF VIRION AN
The genomie DNA of CaMV (Xinjiang isolate) was mapped on the virion DNA with anumber of restriction endonueleases by do......
STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRO
When the R. meliloti (Rm) nifA’-lacZ fusion-carrying plasmids were introduced intothe strains of Agrobacterium tumefaci......
The 3.1 kb BamHI fragment containing the chloroplast rbcL gene from grape (Vitis vinifera L) hasbeen cloned and its res......
Yeast high stable plasmid vector pHC11 was constructed by introducing pEMBL Yi27 cleaved with SmaI into the SnaBI site ......
The promoter region of cauliflower mosaic virus (CaMV) 35s RNA was employed to construct an intermediate expression vect......
作者从受乙型肝炎病毒(HBV)感染的黑猩猩肝中提取了RNA,亦从含有HBV DNA的大鼠细胞系中制备了RNA。作者用S1保护实验、核酸外切酶......
免疫球蛋白的易变区(V)和稳定区(C)基因在个体发育中要经过重排。在精子和胚胎的DNA中它们是一些很分散的DNA片段,在分化的细胞中......
作者从人原发性肝细胞癌(HCC)中克隆出一转化DNA,命名为Lca。他们从11个人的HCC组织及一人肝癌细胞株Lif中分别分离出大分子DNA,......
构建基因组文库是分离真核基因,研究基因结构与功能的一个重要手段。为了分离转化生长因子的基因,我们构建了人肺腺癌细胞系LTEP......
运用DNA重组技术对单纯疱疹病毒Ⅰ型(HSV-1)DNA分子的BamHI C片断进行了次级克隆,建立了该基因片断中8个片断的无性繁殖系。同时利......
从国外引进的人白细胞介素4(IL-4)cDNA,系构建于pCD质粒(pCD-huIL-4),转染真核细胞COS7,表达人IL-4。但表达的IL-4水平较低,为提......
将克隆的乙型肝炎病毒(HBV)DNA(ad w亚型)从pA01-HBV质粒中分离出来,重环化以恢复基因结构,再用BglⅡ裂解这个环状的HBV基因组,得......
作者用特异的重组DNA探针确定胎儿性别。这种男性特异的DNA杂交探针取自人染色体的异染色质3.4kb重复顺序。取1.2mg男性染色体DNA......
非典型21-类固醇羟化酶缺乏症是一种常见的、因生后雄激素过剩引起不同体征为特点的皮质醇合成轻度障碍性疾病。本征是因编码21-......
自1985年以来,国内先后有多篇文章报告了HBV官内感染的研究,即在胎儿肝脏提取物和心脏血清中检出HBV标志,为HBV宫内感染提供了理......
运用含人e珠蛋白基因序列的重组质粒Pe1.3的BamHI/EcoRI1.3kb片段作为探针,研究了中国人e珠蛋白基因5’侧序列中HincⅡ位点的多态......
用大肠杆菌HB 101株扩增了含有poliovirus sabin 1型全序列cDNA plasmid。以不同的方法进行提取 polio cDNA(sabin 1)plasmid,转染......
什么是λ基因组工程λ基因组工程(engineering of the ge-nome of the bacteriophage λ)就是在体内及体外定向改造λ DNA的一种......
