plasmid相关论文
Characterization of NDM-5-producing Enterobacteriaceae isolates from retail grass carp(Ctenopharyngo
We aimed to characterize NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou,China.A total of 196 inte......
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Construction of eukaryotic expression plasmid about human retina-derived NT-3 and its expression, se
The human neurotrophin-3(NT-3) DNA was obtained by RT-PCR from retinal tissue.Expression plasmid of NT-3, p EGFP-N1-NT-3......
Extended-spectrum β-lactamases in K.pneumoniae isolated in China carry distinct types of 16S rRNA me
Eighty-four multiresistant K.pneumoniae (MDR-KP) isolates from a Chinese hospital from January to October 2012 were eval......
Multiple genes encoding fluoroquinolone resistance co-located with ESBL genes in non-typhoidal salmo
OBJECTIVES To investigate the molecular epidemiology of genes that confer resistance to fluoroquinolones and extended sp......
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Transconjugation and genotyping of the plasmid-mediated AmpC β-lactamase and extended-spectrum β-lac
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The increasing incidence of multidrug-resistant <i>Klebsiella pneumoniae</i> strains has become a serious global healthc......
Applicability of plasmid calibrant pMON87712 for quantitative detection of the transgenic soybean MO
The transgenic glyphosate-tolerant soybean MON87712 event was developed by the agrochemical and agricultural biotechnolo......
Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot......
The integration pattern and adjacent host sequences of the inserted pMThGH-transgene in the F4 hGH-transgeniccommon carp......
OBJECTIVE Recent studies have shown that hepsin, a type of transmembrane serine protease, is highly upregulated in prost......
Objective:To study the effects of antisense PTEN gene on the growth and invasion of glioma cells. Methods: A pcDNA3. 1/H......
In order to construct plasmid of hypoxia-inducible factor-lalpha (HIF-1α), and transfect into human lung cancer cells A......
Nasopharyngeal carcinoma (NPC) is one of thehighly prevalent malignancies in SoutheastAsia.1 Chemotherapy and radiothera......
Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hep
AIM: To reverse the multidrug resistance (MDR) by RNA interference (RNAi)-mediated MDR1 suppression in hep-atoma cells. ......
AIM: To explore the effects of ultrasound exposure combined with microbubble contrast agent (SonoVue) on the permeabilit......
Study of nucleophosmin (NPM) gene mutation in patients with acute myeloid leukemia and myelodysplast
Objective To investigate nucleophosmin (NPM) gene mutations in patients with de novo acute myeloid leukemia (AML) with n......
Down-regulation of survivin expression by small interfering RNA induces pancreatic cancer cell apopt
AIM: To investigate the inhibitory effect of small interfering RNA (siRNA) on the expression of survivin in pancreatic c......
To clarify the role of APOBEC3G (A3G) in cellular defense against hepatitis B virus ( HBV), the expression of A3G in nor......
Objective:Cervical cancer has become a major public health problem.The development of effective,systemic therapies for c......
Upregulation of sFlt-1 by Trophoblasts Induces the Barrier Dysfunction of Glomerular Endothelial Cel
This study examined the effect of over-expression of sFlt-1 by trophoblasts on the barrier function of glomerular endoth......
本研究采用SOE-PCR技术,分别获得了CTCF因子结合序列的突变和缺失的序列,构建2个重组质粒pEGFP-N1/1554M和pEGFP-N1/1549。质粒pEG......
目的用PCR方法检测疑似鼠疫现场标本,以建立准确、简便和快速的诊断技术。方法对采自疫源地的自毙鼠标本用两种方法提取DNA,进行ca......
<正> The tumor-inducing plasmid (pTi) of Agrobacterium tumefaciens is a most promising vector system in the genetic engi......
The study of crustacean genetics and breeding has been received more and more at-tention due to the rapid development of......
Prussian blue nanoparticle-loaded microbubbles for photothermally enhanced gene delivery through ult
By adsorbing chitosan(CS)-functionalized Prussian blue(PB) nanoparticles(CS/PB NPs) complexing DNA onto the surface of g......
Under the photocatalysis of 365 nm ultraviolet radiation,ultrafine TiO2 caused the oxidative damage of Teasy plasmid DNA......
Comparison of the virulence plasmid genomes of two strains of Shigella which lost the ability to bin
We determined and analyzed the Shigella flexneri serotype 5 (pSF5) and S. dysenteriae serotype 1 (pSD1) virulence plasmi......
Preparation and Targeting Evaluation of Recombined Intrakine Plasmid pEGFP/SDF-1/KDE/Folate-Liposome
Objective:To prepare folate liposome complexes of recombinant plasmid pEGFP/SDF-1/KDEL wich contained intrakine SDF-1/KD......
Objective: Tea polyphenols present in green tea show cancer chemopreventive effects in many tumor models. Epidemiologica......
目的:克隆和表达G蛋白竞争性抑制肽(GCIP)基因,并研究其抗心肌肥大活性。方法:克隆GCIP基因,采用RTS500 系统表达,镍螯合亲和层析......
Template-module assembly to prepare low-molecular-weight gene transport system with enhanced transme
Based on specific host-guest interactions between amine-modified ?-cyclodextrin(CD-TAEA) and functional adamantane(AD) d......
Bacillus thuringiensis(B.thuringiensis) is a soil-dwelling Gram-positive bacterium and its plasmid-encoded toxins(Cry) a......
Extract from Phyllanthus urinaria L.Inhibits Hepatitis B Virus Replication and Expression in Hepatit
Objective:To explore the effects of the extract from Phyilanthus urinaria L.on hepatitis B virus(HBV) replication and ex......
This study was conducted to build a recombinant strain with highly insecticidal activity and a wide host range by using ......
Multi-technique characterizations of main elements in the vehicle exhaust particles collected in a t
In this study,vehicle exhaust particles were collected three locations (the middle,entrance and outside) of the Dapu Roa......
The composites based on the Ti O2 are potentially used in wetland pollution control. In this work, the biological effect......
Novel pyrazole fused heterocyclic ligands:Synthesis,characterization,DNA binding/cleavage activity a
A series of novel pyrazole fused heterocyclic derivatives were synthesized via a two-step procedure or a one-pot two ste......
Highly Efficient and Economical Baculovirus Expression System for Preparing Human Papillomavirus Typ
To improve the existing human papillomavirus type16 (HPV16) virus-like particle (VLP) preparation, a highly efficien......
5-Aminolevulinic acid(ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide appli......
DRUG AND PLASMID DNA CO-DELIVERY NANOCARRIERS BASED ON ABC-TYPE POLYPEPTIDE HYBRID MIKTOARM STAR COP
We report on the fabrication of self-assembled micelles from ABC-type miktoarm star polypeptide hybrid copolymers consis......
通过二代和四代聚酰胺-胺树枝状聚合物(G2和G4表示)末端分别修饰苯丙氨酸和色氨酸合成了多种新型聚合物载体,进而考察了其与质粒的......
Objective:The aim of this study was to investigate whether astrocyte elevated gene 1 (AEG1) regulates COX-2 expression i......
Screening of genes for proteins interacting with the PS1TP5 protein of hepatitis B virus:probing a h
Background The hepatitis B virus (HBV) genome includes S, C, P and X regions. The S region is divided into four subregio......
目的:研究白介素-10(IL-10)对实验性自身免疫性甲状腺炎小鼠的基因治疗作用。方法:将IL-10质粒DNA注射入由猪甲状腺球蛋白(pTg)诱......
Comparison of CIM~ C4-HLD monolithic column with Sartobind phenyl membrane column for pIDKE2 purifi
The main component of the Center for Genetic Engineering and Biotechnology(CIGB)candidate vaccine against Hepatitis C vi......
AIM:Transfer and expression of insulin gene in vivo arean alternative strategy to improve glycemic control in type1 dia......
