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将克隆的乙型肝炎病毒(HBV)DNA(ad w亚型)从pA01-HBV质粒中分离出来,重环化以恢复基因结构,再用BglⅡ裂解这个环状的HBV基因组,得到包括转录所需的启动子和多腺苷酸位点的完整的HBsAg基因.把它连接到含69%的牛乳头状瘤病毒(BPV)的BamHI-HindⅢ转化区的pBPV_(T69)载体质粒
The cloned hepatitis B virus DNA (adw subtype) was isolated from the pA01-HBV plasmid, re-circularized to restore the gene structure, and then the circular HBV genome was cleaved with BglII to obtain Of the promoter and polyadenylation sites of the HBsAg gene was ligated into the pBPV_ (T69) vector plasmid containing 69% of the BamHI-HindIII transformation region of bovine papilloma virus (BPV)