cloned相关论文
Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropen
Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive gen......
Specific T-cell Responses to CFP10,an Secreted Antigens of Mycobacterium Tuberculosis Protein,in Chi
Objective To construct prokaryotic expression vector of CFP-10 gene,and obtain recombinant protein,and the recombinant C......
Rice grain shape,grain length(GL),width(GW),thickness(GT)and length-to-width ratio(LWR),are usually controlled by multip......
Grain yield is one of the most important indexes in rice breeding,which is governed by quantitative trait loci (QTLs).Di......
Expression and Characterization of a Thermostable Acyl-peptide Releasing Enzyme ST0779 from Sulfolob
Acyl-peptide releasing enzyme(AARE) belongs to a serine peptidase family and catalyzes the NH2-terminal hydrolysis of N......
Expression and Characterization of a Sigma-Class Glutathione S-transferase of the Oriental Migratory
A cDNA encoding a sigma-class glutathione S-transferase of the locust, Locusta migratoria manilensis (LmGSTs1), was clon......
In recent years, Galium aparine L. has not been controlled by tribenuron-methyl in major Chinese winter wheat fields. Th......
Objective: To study the relationship of the mutation of HBV preS/S gene in HBsAg carrying pregnant women and intrauterin......
AIM:To investigate the role of soluble Fas ligand in autoimmunediseases.METHODS:RT-PCR was performed to amplify sFasL c......
AIM:To investigated the effects of the SNPs (rs411021,rs436045,rs427811,rs385039 and rs809912) on geneexpression and fu......
Objective To study the alternative expression and sequence of human elongation factor-1 (human EF-1 p31) during malignan......
Recombinant outer membrane protein F-B subunit of LT protein as a prophylactic measure against Pseud
AIM: To study immunogenicity of outer membrane protein F(Opr F) fused with B subunit of LT(LTB), against Pseudomonas aer......
Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile
Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somati......
The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and ......
Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like ......
Charcot-Marie-Tooth(CMT) disease is the most common inherited peripheral neuropathic disorder.CMT is clinically and gene......
Synthesis and evaluation of monoclonal antibody against Plasmodium falciparum merozoite surface anti
Objective:To assess the quality of expressed MSP-2 and also to confirm the immune response against different domains of ......
Domain Ⅲ of Dengue Virus Serotype 2 Envelope: Expression at High Levels in Escherichia coli and Comp
Obejective The domainⅢof dengue virus type 2 envelope was cloned and expressed in Escherichia coli and the inhibited ef......
Immunological characterization of recombinant Wuchereria bancrofti cuticular collagen(COL-4)as putat
Objective:To elucidate immunoprophylactic potential of recombinant Wuchereria bancrofti(W.bancrofti)cuticular collagen(C......
Construction of a recombinant plasmid pGEX-Sj26GST of Schistosoma japonicum and expression in Escher
Objective To construct and express a recombinant plasmid pGEX-Sj26GST of Schistosoma japonicum(Sj)in Escherichia coli(E.......
A unique insertion of low complexity amino acid sequence underlies protein-protein interaction in hu
Objective:To investigate the multienzyine complex formation of human malaria parasite Plasmodium falciparum[P.falciparum......
Ethylene has been implicated as a sex-determining hormone in cucumber. Its exogenous application increases femaleness,an......
(Pro)renin receptor [(P)RR], a receptor for renin and prorenin, was first cloned in 2002. Since then, the pathophysiolog......
Melon downy mildew caused by Pseudoperonospora cubensis leads to significant losses in melon yields worldwide. Reverse-t......
OBJECTIVES: To construct a DNA vaccine capable of expressing the S gene of hepatitis B virus (HBV)and evaluate the expr......
Based upon analysis of data obtained from theATH1 microarrays, a cDNA that was highly induced afterdrought treatment, wa......
OBJECTIVE To investigate whether micro RNA (miRNA) miR-21 regulates dimethylarginine dimethylaminohydrolase 1 (DDAH1) ex......
Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase c
Xyloglucan endotransglycosylase is an identified relaxation factor with functions of easing and extending of plant cell ......
Purpose:To clone the vanabie region gene ot light chain ot monoclonal antibody against human retinoblastoma and to anal......
DIFFERENTIATION AND MALIGNANT SUPPRESSION INDUCED BY MOUSE ERYTHROID DIFFERENTIATION AND DENUCLEATIO
To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in ou......
OPM_(05)-M_(2100),the specific RAPD fragment of Bursaphelenchus xylophilus,was collected from agarose gels and purified.......
Cloning and Bioimformatic Analysis of Cellulose Synthase-like Protein Gene,CICsID1 from Cunninghamia
The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the ......
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other r......
The M2 protein from influenza A functions as a proton channe1. It has been cloned andoverexpressed in Escherchia coli. h......
AIM:To construct a recombined human AFP eukaryoticexpression vector for the purpose of gene therapy and targettherapy o......
AIM:APMCF1 is a novel human gene whose transcriptsare up-regulated in apoptotic MCF-7 cells.In order to learnmore about......
AIM:A single-chain antibody fragment,ND-lscFv,againsthuman colorectal carcinoma was constructed and expressedin E.coli,......
Summary:To construct the antisense transforming growth factorβ1(TGFβ1)gene and investigatethe effect of TGFβ1 autocr......
Cancer cells escape from growth cont rol by accumulating genetic and epig enetic alterations.In rare instances,epigeneti......
To study the effect of ntrC gene product on the expression and regulation of other important nitrogen-fixing genes in A......
Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their ex
The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the ......
Molecular Cloning and Sequence Analysis of a Tonoplast Aquaporin Gene TIP1 Associated with Tapping P
[Objective]To address the role of aquaporins(AQPs)in the occurrence of Tapping Panel Dryness(TPD),a phenomenon with tapp......
AIM:Human cytochrome P-450 2E1(CYP2E1)takes part inthe biotransformation of ethanol,acetone,many small-molecule substra......
To obtain human sodium/iodide symporter gene cDNA for studying its potential ability as a radioiodide treatment fo......
Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with diffe
AIM:In order to elucidate the molecular mechanism oflymphatic metastasis of hepatocarcinoma,we detectedthe difference o......
Human endostatin gene transfer,either naked or with liposome,has the same inhibitory effect on growt
AIM:To explore a safe and efficient strategy of tumortherapy using anti-angiogenetic agents.METHODS:Endostatin gene wit......
Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastri
AIM:To investigate the association of TNF polyrnorphisrnswith chronic atrophic gastritis (CAG) and gastric adenocarcin-......
AIM:Over 90% of drugs are metabolized by the cytochromeP-450 (CYP) family of liver isoenzymes.The most importantenzymes......
Cloning, Expression, Purification and Crystallization of NHR3 Domain from Acute Myelogenous Leukemia
The t(8;21) translocation is one of the most frequent chromosome abnormalities in acute myeloid leukemia. This transloc......
Genetic alterations of hepatocellular carcinoma by random amplified polymorphic DNA analysis and clo
AIM: To study the genetic alterations and their association with clinicopathological characteristics of hepatocellular c......