CaMV相关论文
STUDY ON THE GENOME OF CAULIFLOWER MOSAIC VIRUS (XINJIANG ISOLATE)——RESTRICTION MAPPING OF VIRION AN
The genomie DNA of CaMV (Xinjiang isolate) was mapped on the virion DNA with anumber of restriction endonueleases by do......
The promoter region of cauliflower mosaic virus (CaMV) 35s RNA was employed to construct an intermediate expression vect......
近几年来,通过基因工程技术对改造病毒变害为益,或通过它创建某些新的有用产品和新的生命类型以更有效地服务于人类的需要,并进一......
大白菜[Brassica campestris L.ssp.pekinensis(Lour) Olsson]是一种中国的古老蔬菜作物,广泛栽培于远东各国。在美国,大白菜是最......
近年来,武汉郊区蔬菜病毒病日趋严重,尤以十字花科菜蔬及番茄、辣椒为甚。国外报道侵染十字花科的病毒主要是黄瓜花叶病毒(CMV)、......
从污泥中分离得到光合细菌球形红假单胞菌P_4株。实验结果表明喷洒P_4菌株培养液能够提高蚕豆与苋色藜植株抵抗黄瓜花叶病毒(CMV)......
Expression of ORF2 partial gene of hepatitis E virus in tomatoes and immunoactivity of expression pr
AIM:To transfer hepatitis E virus(HEV)ORF2 partial geneto tomato plants,to investigate its expression in transformantsa......
The AC2 gene of cotton leaf curl virus (CLCuV) was obtained by polymerase chain reaction (PCR) . The total DNA of the C......
Expressing activity of promoter elements of large intergenic region from cotton leaf curl virus in h
Cotton leaf curl virus (CLCuV) is a type of single-stranded DMA virus, belonging to geminivirus of subgroup Ⅲ. In order......
棉花曲叶病毒 (CLCuV)是一种单链DNA病毒 ,属于双生病毒亚组Ⅲ。检测了双生病毒双向启动子在根癌土壤杆菌 (Agrobacteriumtumefaci......
A Transient Expression System for the Functional Assessment of Early Response Genes on the Powdery M
The principle and the basic steps of the transient assay system for the functional assessment of early response genes on......
Impact Evaluation of Insect-Resistant Transgenic Rice on the Feeding and Oviposition Behavior of Its
The feeding and oviposition behavior of the brown planthopper (BPH), Nilaparvata lugens on two transgenic indica ric......
针对花椰菜花叶病毒(CaMV)35S启动子的保守序列设计特异性引物,并进行条件优化,建立CaMV35S启动子的LAMP检测方法。该方法具有良好......
将烟草中经修饰的Ⅰ类几了质酶和β－1，3-葡聚糖酶cDNA分别加上CaMV35S启动子和NOS终止子，依次插入到同一植物表达载体pBin19上，获得植......
Expression of a Carrot 36 kD Antifreeze Protein Gene Improves ColdStress Tolerance in Transgenic Tob
Antifreeze proteins (AFPs) enable organisms to survive under cold conditions, and have great potential in improv- ing co......
以转化CaMV弱株系Bari 1基因Ⅵ的大白菜、菜薹、紫菜薹和花椰菜植株为试材 ,研究了CaMV弱株系Bari 1基因Ⅵ所提供的遗传工程交叉保......
日本等国的科学家利用基因工程方法培育抗冷的作物品种已取得较大的进展。植物抗冷的关键是叶绿体对冷凉的抗性,而叶绿体对冷的敏......
植物抗虫基因工程是解决农业生产中虫害问题的一种重要手段,利用抗虫基因使植物获得抗虫性已经得到了实际应用.在可以利用的抗虫基......
Construction of Two Gateway-compatible Destination Vectors for Tomato Functional Genomics under Abio
[Objective] This research aimed at constructing two gateway-compatible plant expression vectors for functional genomics ......
To obtain a suitable condition for electroporation transformation in indica rice, the 10-day-old immature embryos were s......
以油菜内源基因PEP、抗除草剂基因(BAR、PAT)、筛选基因NPTII、常见启动子(CaMV35S、FMV35S)和终止子NOS为检测对象,通过研究不同......
第三讲介绍了用 Ti 质粒为载体,可以将外源基因转入植物。尽管还没有育成新的作物,但这是一个预兆,表明科学技术发展到今天,人类......
Bar gene driven under the control of CaMV35s promotor was delivered into the immatureembroys of a japonica rice cultiva......
Production of herbicide resistant transgenic rice plant from immature embryos using biolistic method
Plasmid pCB1,which contains a bar gene un-der the control of CaMV 35S promoter,hadbeen successfully transformed into imm......
A total of 210 soybean samples collected from different areas of Heilongjiang Province were analyzed by PCR to detect th......
Science Letters:Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnorma
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to ......
Maize Lls1 (lethal leaf-spot 1) gene and its Arabidopsis orthologue AtAcd1 have been sug-gested to encode pheide a oxyge......
Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS} alters the height of t
Cellular apoptosis susceptibility (CAS) gene plays important roles in mitosis, development and export of importin a from......
The pollen tube pathway method of transformation has been reported to be successful in most crops,but less successfu in ......
A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promote......
The foxtail millet Si69 gene is a Wali7 (wheat aluminum-induced protein 7) homologue and may functio
We isolated a clone, named Si69, from a foxtail millet immature seed cDNA library. The protein encoded by Si69 contains ......
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping......
以甘蓝型油菜‘德油五号’基因组DNA为模板,通过反向PCR扩增得到肌醇半乳糖苷合成酶基因(BnGOLS1)启动子片段,长度为827bp。PLACE......
为了研究凤梨科植物开花的机理,本研究利用5’RACE和3’RACE方法从蜻蜓凤梨中克隆得到响应开花的主要调控基因FT1。该基因CDS序列......
细胞分裂素在植物生长发育的许多方面行使重要功能,异戊二烯转移酶(IPT)是细胞分裂素合成的关键酶。利用生物信息学和RACE、TAIL技......
根据麻疯树MIPS基因序列,设计特异性的巢式引物,运用TAIL-PCR法两次步移得到MIPS基因5’端侧翼序列,序列分析显示含有多个胁迫应答......
高粱是世界上仅次于小麦、水稻、玉米和大豆的重要作物之一,然而由于其高效、稳定的遗传转化体系的建立较难,限制了其转基因研究进......
糖苷生物碱(steroidal glycoalkaloids,SGAs)是一类存在于茄科和某些百合科植物的重要次生代谢物,与植物的抗逆性和产品品质有密切......
Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation
Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato.The bi......
以矮牵牛为试材,通过根癌农杆菌介导ACC脱氨酶基因(ACDS)(分别由CaMV35S、SAG12和CHSA启动子驱动)转化矮牵牛叶盘,探讨了预培养时......
本研究通过对茄科植物的E8基因的非编码区DNA序列进行亲缘关系及功能元件保守性分析,发现E8基因的非编码区包含ERE,茉莉酸和低温干......
AcSERK1是菠萝(Ananas comosus)体细胞胚发生初期特异表达的基因,为探讨其转录调控规律,对其5′上游调控序列的转录起始位点及启动......
实验构建了CaMV35S启动子控制下的大豆抗病相关基因SR1的正反义植物双元表达载体pBISR1(+)和pBISR1(-).通过根癌农杆菌叶盘转化法,......
用CTAB法提取转基因大豆(Roundup Ready品系)的总DNA.根据行业标准SN/T 1195-2003,合成用于扩增花椰菜花叶病毒(Cauliflower mosai......
