pBluescript相关论文
RHEUMATOID arthritis (RA) is an autoimmune disease which progressively and systematicallyinvades the joints of bones. A......
目的在毕赤酵母中表达重组人ADAM15去整合素区域蛋白(recombinant human disintegrin of ADAM15,rhddADAM15)与人血清白蛋白融合蛋......
Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of va
AIM: To investigate the killing efficiency of a recombinant plasmid containing a thymidine kinase (TK) domain insert dri......
The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima (Vahl.) Fern was constructed ......
【目的】构建溶磷黑曲霉H1的cDNA文库,并从中筛选溶磷相关基因。【方法】利用SMART技术合成黑曲霉H1的双链cDNA并将其连接于pBlues......
目的 :构建用于小鼠牙本质涎磷蛋白基因敲除的载体 ;方法 :PCR获得上、下游两条同源臂 ,将同源臂和正、负双向筛选标志按一定顺序......
AIM:To construct a vacA-knockout Helicobacter pylorimutant strain,whose only difference from the wild strain isits disr......
副结核分枝杆菌为Johne病的病原体,含有一种称为A36的主要抗原复合物。该复合物内的34 000蛋白质为免疫功能区,是一种独特的蛋白......
构建了大肠杆菌一分枝杆菌穿梭质粒pBCG-2000,并将日本血吸虫26ku抗原(Sj26GST)基因在分枝杆菌中进行了克隆和表达.以质粒pBluescr......
随着单纯疱疹病毒(HSV)感染发病率的不断上升,HSV防治也成为性传播疾病防治的重要方面。HSV疫苗不仅可以预防HSV感染,而且还具有免......
【目的】扩增日本血吸虫 2 6ku谷胱甘肽硫 转移酶 (Sj2 6GST)基因片段 ,构建其重组原核载体 ,以研制SjGST重组克隆。【方法】根据......
目的克隆人软骨来源形态发生蛋白 - 1(CDMP - 1)成熟肽编码区的基因片段。 方法用异硫氰酸胍一步法从人软骨组织和酶消化法原代培......
为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA......
Objective To study the role of cyclinD1 and CDK4 in malignant transformation of human fetal lung diploid fibroblast cell......
cDNA microarray in isolation of novel differentially expressed genes related to human glioma and clo
Background This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA......
目的 既往研究表明LRP16基因是一个雌激素反应基因,其表达水平与乳腺癌细胞增殖及侵袭密切相关.为对该基因进行生理功能研究,本文......
To clone and sequence the human tissue kallikrein gene of Chinese, and to construct eu- karyotic expression recombinant ......
This study focuses on the expression of human protein tyrosine phosphatase 1B(PTP1B) catalytic domain(ΔPTP1B) and prepa......
Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oo
AIM: To study the effects of acetazolamide and anordiol on osmotic water permeability in aquaporin 1 (AQP1)-cRNA injecte......
目的:构建人生存素(survivin)的多表位真核表达载体,并在人树突状细胞(DC)中进行表达。方法:人工合成含4个survivin的HLA-A2类限制......
Actins are ubiquitous to all of the known eukaryotic organisms. Since the firstdiscovery of actins in higher plants by ......
目的：从重组人Ｂｃｌ－２质粒中制备对基因重组和ＤＮＡ测序等有用的ｐＢｌｕｅｓｃｒｉｐｔⅡＫＳ（－）载体。方法：先将重组人Ｂｃｌ－２质粒转化ＤＨ５α菌株，小规模制备此质粒ＤＮＡ，然后从低熔点琼脂糖凝胶......
