Objective:To explore the effects of the extract from Phyilanthus urinaria L.on hepatitis B virus(HBV) replication and expression in HBV transient transfection model in vitro.Methods:The eukaryotic expression plasmid pHBV1.1,which contains 1.1-fold-overlength genome of HBV,was transfected into the human hepatoma cell line,HepG2,to establish and assess the HBV transient transfection model.The extract from Phyilanthus urinaria L.was prepared in different concentrations and methyl thiazolyl tetrazolium was used to detect the maximum nontoxic concentration of the drug.The extract from Phyilanthus urinaria L.were added into the transfected cell,at the concentrations of 0.8,0.2 and 0.05 g/L,respectively.Four days after drug application,enzyme-linked immuno sorbent assay was used to detect the concentration of HBsAg in the supematants,Southern blot was applied to analyze HBV DNA level,and Western blot was used to detect the expression of HBcAg in cells.Results:After the transfection of plasmid pHBV1.1 into HepG2 cells,the concentration of HBsAg in supematants was increased obviously as compared with that of the normal cells(P<0.05),and all expected HBV replicative intermediates were confirmed by Southern blot analysis,which ensured the successful establishment of the HBV transient transfection model.After the application of drugs at the concentrations of 0.8 and 0.2 g/L,the level of HBsAg was obviously decreased in the supematants,as compared with that of the virus group(P<0.05);Southern blot showed that the level of HBV rc DNA,ds DNA,ss DNA was obviously reduced compared with that of the virus group(P<0.01);Western blot revealed that the expression of HBcAg in the drug group was obviously inhibited,as compared with that of the virus group(P<0.01).Conclusions:The extract from Phyilanthus urinaria L.obviously inhibited replication and expression of HBV in HBV transfected cell lines in vitro,thus exerting distinctive anti-HBV effects. Objective: To explore the effects of the extract from Phyilanthus urinaria L. on hepatitis B virus (HBV) replication and expression in HBV transient transfection model in vitro. Methods: The eukaryotic expression plasmid pHBV1.1, which contains 1.1-fold-overlength genome of HBV, was transfected into the human hepatoma cell line, HepG2, to establish and assess the HBV transient transfection model. The extract from Phyilanthus urinaria L. was prepared in different concentrations and methyl thiazolyl tetrazolium was used to detect the maximum nontoxic concentration of the drug. The extract from Phyilanthus urinaria L. were added into the transfected cell, at the concentrations of 0.8, 0.2 and 0.05 g / L, respectively. Flow days after drug application, enzyme-linked immuno sorbent assay was used to detect the concentration of HBsAg in the supematants, Southern blot was applied to analyze HBV DNA level, and Western blot was used to detect the expression of HBcAg in cells. Results: After the transfection of plasmid pHBV1.1 into HepG2 cells, the concentration of HBsAg in supematants was increased obviously compared to that of the normal cells (P <0.05), and all expected HBV replicative intermediates were confirmed by Southern blot analysis, which established the successful establishment of the HBV transient transfection model. After the application of drugs at the concentrations of 0.8 and 0.2 g / L, the level of HBsAg was significantly decreased in the supematants, as compared with that of the virus group (P <0.05); Southern blot showed that the level of HBV rc DNA, ds DNA, ss DNA was significantly reduced compared with that of the virus group (P <0.01); Western blot revealed that the expression of HBcAg in the drug group was obviously inhibited, as compared with that of the virus group (P <0.01) .Conclusions: The extract from Phyilanthus urinaria L.obviously inhibited replication and expression of HBV in HBV transfected cell lines in vitro, thus exerting distinctive anti-HBV effects.