luciferase相关论文
Construction of optical reporter genes for cell cycle G1 and S phase, and their applications for mon
Background: Cell cycle is the process that from the beginning of previous mitosis end to next mitosis complete. Studies ......
Anti-CD3×EGFR bispecific antibody redirects cytokine-induced killer cells to glioblastoma in vitro a
The epidermal growth factor receptor(EGFR)is an attractive target for immunotherapy of EGFR+tumors....
A complete set of new phospate-caged photolabile nucleoside phosphoramidites were synthesized,and were then site-specifi......
Tuning the color of firefly bioluminescence:a theoretical study on luciferin and luciferase modifica
Firefly bioluminescence has various applications,such as biological analysis.To enhance the detection sensitivity and ex......
We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral transl......
设计合成含有苯并噻唑及嘧啶并噻唑母核的化合物,评价其对信号转导及转录激活因子3(STAT3)信号转导通路的影响.以实验室前期报道的......
AIM:To investigated the effects of the SNPs (rs411021,rs436045,rs427811,rs385039 and rs809912) on geneexpression and fu......
Molecular cloning of promoter in human fibrinogenlike protein 2 (hfgl2) gene and functional analysis
The aim of this study is to investigate the important regulative elements region which plays an important role on the ac......
Gene transcription mechanisms are critical control points for cell function and differentiation as well as disease patho......
Objective To observe the effects of HCV protein,NS3 and NS5A on IFN-βin HepG2 cells and its regulation mechanism.Method......
Functional single-nucleotide polymorphisms in the SCGB3A2 promoter are associated with susceptibilit
Objective To investigate the association of functional single-nucleotide polymorphisms(SNPs) in the SCGB3A2 promoter wit......
AIM: To investigate the potential for early gestation placenta-derived mesenchymal stromal cells(PMSCs) for fetal tissue......
Construction of adenoviral vector for luciferase driven by hTERT core promoter modified with MYC-res
AIM:To construct adenoviral vectors for luciferase driven by human telomerase reverse transcriptase(hTERT) core promoter......
Characteristics and application of established luciferase hepatoma cell line that responds to dioxin
AIM:To establish a luciferase reporter cell line that respondsdioxin-like chemicals (DLCs) and on this basis to evaluat......
目的 构建AFP增强子CMV启动子调控下的HSV -TK真核表达质粒用于肝细胞癌的靶向基因治疗。方法 采用PCR方法从HepG2细胞基因组DNA......
AIM:Most cancer cells acquire immortal capability bytelomerase activation.The human telomerase reversetranscriptase gen......
目的 构建萤火虫荧光素酶(FFLuc)重组表达的结核分枝杆菌菌株,利用其建立抗结核药物最低抑菌浓度(MIC)检测方法,并应用该方法测定......
目的 克隆人肾组织γ-谷氨酰基羧化酶(cGCX)基因5'侧翼启动子区,并对其转录活性进行分析.方法 对GGCX基因5'侧翼区进行生物信息学......
A series of LTR deletion-directed Luc expression plasmids were constructed and transfected into Jurkat cells with or wi......
Objective:To investigate the effects of -2242,-1892 and -1837 single nucleotide polymorphisms(SNPs) on toll-like recepto......
Hepatitis C virus NS3/4A with sequence variation at amino-terminus has different serine protease act
Objective: To construct the point mutation plasmids expressing HCV NS3/4A with different secondary structures at the N-t......
AIM:To elucidate the effect of antioxidants,resveratrol (RVT)and astaxanthin(AXN),on hepatitis C virus(HCV) replication.......
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RNR编辑(RNA editing)是RNA转录后的一个自然过程,经过编辑修饰的RNA使遗传编码上的误差得以校正。对于基因点突变引起的遗传性疾......
RNR编辑(RNA editing)是RNA转录后的一个自然过程,经过编辑修饰的RNA使遗传编码上的误差得以校正。对于基因点突变引起的遗传性疾......
Objective To study the regulatory mechanism of SATB1 repression in cells other than T cells or erythroid cells, which ha......
Improvement of Chemically-activated Luciferase Gene Expression Bioassay for Detection of Dioxin-Iike
Objective To improve the chemically-activated luciferase expression (CALUX)bioassay for detection of dioxin-like chem......
目的:通过改造炭疽毒素保护性抗原Protective Antigen(PA)及致死因子Lethal Factor(LF),尝试建立更加广谱的新型炭疽毒素靶向给药......
目的探讨mi R-203对胞质多聚腺苷酸化元件结合蛋白4(cytoplasmic polyadenylation element binding protein4,CPEB4)基因的靶向作......
目的 :探讨miR-30a在人乳腺癌细胞系中的表达,及其对乳腺癌细胞系增殖、迁移和侵袭的影响,并寻找作用靶基因。方法:用q RT-PCR法检......
Androgen receptor coregulator ARA267-a interacts with death receptor-6 revealed by the yeast two-hyb
ARA267-a is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, u......
AIM:To evaluate the inhibition effect of HCV NS5A on p53transactivation on p21 promoter and explore its possiblemechani......
Inhibitor RNA blocks the protein translation mediated by hepatitis C virus internal ribosome entry s
AIM:To investigate the inhibitory effect of hepatitis C virusinternal ribosome entry site (HCV IRES) specific inhibitor......
AIM: To discover compounds which can regulate Klotho promoter activity. Klotho is an aging suppressor gene. A defect in ......
Bone morphogenetic protein-6 (BMP-6), which belongs to the transforming growth factor-β (TGF-β) superfamily, is a mult......
研究背景树突状细胞(DCs)是体内功能最强大的抗原递呈细胞,具有独特的激活初始T淋巴细胞的功能,是启动和调控特异性免疫应答的中心......
The autosome dominant disorders, ring dermoid of the cornea (RDC), iris hypolasia (IH) and Axenfeld-Rieger syndrome (ARS......
Objective To investigate the impact of glucose metabolism-related protein 1(GMRP1)on the regulation of c-Myc gene transc......
Objective To investigate whether α-hemoglobin stabilizing protein (AHSP), the α-globin-specific molecular chaperone, i......
Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen ......
Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen ......
Objective To investigate the regulatory mechanisms of acetylated p53 in the expression of microtubule-associated protein......
The process of identifying novel human immunodeficiency virus 1 (HIV-1) inhibitors presents a challenge for industrial a......
miRNA在动物及人类中广泛存在,并且与发育、分化、凋亡、脂肪代谢、病毒感染和癌症等多种重要生物学过程有联系。虽然在不同的时空......
为观察nsP2-726Pro定点诱变对辛德毕斯病毒(Sindbis virus,SINV)XJ-160复制子载体特性的影响,以XJ-160病毒复制子载体pBRepXJ为分......
