scFv相关论文
＜正＞ Mycotoxin deoxynivalenol produced by Giberella zeae is toxic both to human and animals. It is also a virulence factor......
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Determination of scFv fragments number displayed on the surface of m13 phage by capillary electropho
Phage display technology is a powerful tool to find ligands with high affinities to given targets.Arguably the most succ......
A Single Chain Antibody using LoxP511 as the Linker Enables Large-Content Phage Library Construction
To obtain natural or "me-better" antibodies (e.g., affinity-maturated antibodies), phage display libraries are widely ......
利用蛋白酶解法从完整抗体制备Fv片段是个费时费力的繁琐过程，且对于多种McAb的Fv片段制备是不成功的。利用PCR方法，从抗体产生细胞......
The use of human T lymphocytes genetically modified to express chimeric antigen receptors on their surfaces has emer......
Zinc transporter 8(ZnT8) is a major autoantigen and a predictive marker in type 1 diabetes(T1D). To investigate ZnT8-spe......
AIM To develop the single chain variable fragment ofMG_7 murine anti-human gastric cancer monoclonalantibody using the ......
抗独特型抗体能够模拟肿瘤抗原,调节机体免疫功能,已成为肿瘤免疫治疗研究中一个十分重要的研究领域.本中心制备的卵巢癌抗独特型......
本文用ＳＬＥ病人外周血淋巴细胞抽提ＲＮＡ，采用ＲＴＰＣＲ反应以人的特异性ＨｕＶＨＢＡＣＫ、ＨｕＪＨＦＯＲ和ＨｕＶＬＢＡＣＫ、ＨｕＶＬＦＯＲ引物扩增人抗体的ＶＨ和ＶＬ基因片段，并将其与ｌｉｎｋｅｒ拼接成ＳｃＦｖ片段克隆于大肠杆菌，使其与......
One concept for immune therapy of cancer involves induction of antigen mimic antibodies to trigger the immune response a......
目的构建并在CHO细胞中表达抗人CD86单链抗体(CD86-scFv),研究该抗体与抗原的结合特性及介导的生物学功能。方法从分泌鼠源CD86抗......
目的:制备靶向表皮生长因子受体(epithelial growth factor receptor,EGFR)隐蔽表位(287-302)的免疫毒素,并鉴定其生物学功能。方......
Screening and identification of human Zn T8-specific single-chain variable fragment (scFv) from type
Zinc transporter 8(ZnT8) is a major autoantigen and a predictive marker in type 1 diabetes(T1D). To investigate ZnT8-spe......
目的 :从半合成噬菌体抗体库中克隆抗地高辛 (Dig)人单链抗体 (ADAScFv) ,为Dig中毒的诊断和治疗提供人源性抗体。方法 :①以改良......
目的构建抗肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)单链抗体(Single chain fragment variable,scFv)的原核高效表达体......
目的探究抗人死亡受体5(DR5)单链抗体(aDR5ScFv)的特异性。方法 ELISA检测aDR5ScFv的效价、亲和力和表位分析。Western blotting检......
Intracellular expression of a single-chain antibody directed against type IV collagenase inhibits th
It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in ......
目的 :构建一个鼠源性的抗内毒素单链噬菌体抗体库 ,从中筛选出对内毒素具有较高亲和力的单链抗体。方法 :从小鼠脾细胞中提取总RN......
Identification of tumor associated single-chain Fv by panning and screening antibody phage library u
AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment(ScFv)of......
目的 构建胶质瘤患者人源性噬菌体抗体基因库。方法 从脑胶质瘤患者外周血淋巴细胞（PBL）中提取细胞总RNA,经逆转录后用套式多聚酶链......
PRODUCTION OF PHAGE-DISPLAYED ANTI-IDIOTYPIC ANTIBODY SINGLE CHAIN VARIABLE FRAGMENTS TO MG7 MONOCLO
To generate phage-displayed anti-idiotypic antibody single chain variable fragments (anti - Id ScFv) to MG7 monoclonal a......
采用重组技术,通过一种设计和构建方法可研制出人类抗体。作者应用V基因噬菌体显示技术,研制出了一种针对HPA-1a的人类单链可变功......
AIM:A single-chain antibody fragment,ND-lscFv,againsthuman colorectal carcinoma was constructed and expressedin E.coli,......
Construction, expression and characterization of the engineered antibody against tumor surface antig
The c-erbB-2 proto-oncogene encodes a 185kDa protein p!85, which belongs to epidermal growth factor receptor family. Am......
目的 制备抗人结肠癌单抗MC5的具功能活性的可溶性单链可变区片段 (ScFv)。方法 从分泌MC5的杂交瘤细胞分离mRNA ,RT PCR分别扩......
AIM:To obtain human recombinant Fv-immunotoxin hscFv_(25)-mTNFα(mutant human TNFαfused to human scFv_(25))againsthepa......
AIM:To construct a phage display library of human single-chain variable fragment (scFv) antibodies associated withesoph......
Tumor Antigen Specific Activation of Primary Human T-Cells Expressing a Virally Encoded Chimeric T-C
We have developed and tested chimeric T-cell receptors (TCR) specific for p185HER2. In these experiments, retroviral vec......
肿瘤相关性抗原 (TAAs)是在肿瘤细胞内或肿瘤细胞表面的大分子 ,在正常组织中是不可见的或较少集中存在的。目前有许多针对肿瘤进......
Human scFv antibody fragments specific for hepatocellular carcinoma selected from a phage display li
AIM: To identify the scFv antibody fragments specific for hepatocellular carcinoma by biopanning from a large human naiv......
目的:构建及表达抗人CD33单链抗体(抗CD33-scFv)基因,并检测其生物活性.方法:采用RT-PCR方法从分泌抗人类白细胞表面分化抗原CD33......
克百威(carbofuran,CBF),又名呋喃丹,属于氨基甲酸酯类农药,广泛应用于粮食、蔬菜、水果及经济作物等害虫的防治。克百威对人、畜......
引起奶牛乳腺炎的原因复杂多样,金黄色葡萄球菌是其中重要的病原之一。目前生产上针对金黄色葡萄球菌引发的奶牛乳腺炎的防治有抗......
黄绿青霉素(Citreoviridin,CIT)主要是由黄绿青霉菌(Penicillum Citroviridin,PCV)在低温高湿度等环境中产生的一种毒性次生代谢物......
研究背景整合素αvβ3在许多肿瘤中高表达并促进肿瘤进展[1],是肿瘤抗血管治疗的重要靶点[2-5]。其单链抗体(ScFv αvβ3),可在体......
为构建抗黄曲霉毒素M1单链抗体(scFv)基因,并进行蛋白质结构模拟及理化特性分析。采用重叠延伸PCR方法,以能特异性分泌抗黄曲霉毒......
目的：在大肠杆菌高效表达抗胃癌单抗３Ｈ１１的ｓｃＦｖ。方法：利用ＰＣＲ技术将３Ｈ１１ｓｃＦｖ基因从分泌型表达载体转移至高效表达载体，获得３Ｈ１１ｓｃＦｖ包含体的表达，经超声裂解、洗涤......
Expression and identification of recombinant soluble single-chain variable fragment of monoclonal an
AIM:To generate soluble single chain variable fragments(ScFv)of monoclonal antibody MC3 recognizing colorectaland gastr......
AIM:To examine the molecular mass and identify thebioactivity of MG7 scFv for its application as a targetingmediator in......
通过DNA重组技术和PCR技术，将Fab表达载体改建成了ScFv表达载体。该载体可以表面表达方式产生噬菌体抗体，用于抗体库技术，也可以分泌......
目的 筛选、鉴定抗乙型肝炎病毒 (HBV)表面抗原 (HBsAg)蛋白的人源单链可变区抗体 (ScFv)的编码基因 ,为细胞内表达小分子单链抗......
The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis inducedby Sc
Schistosomiasis is one of the most prevalent parasitic diseases in China,and hepatic fibrosis caused by schistosome infe......
