Cas9相关论文
解析大脑中神经集群的活动和相互连接是当今脑科学研究所面临的重大挑战之一。早在2011年,美国西北大学的Konrad P.Kording教授就......
CRISPR-Cas系统为细菌与古生菌的一种免疫防御机制。基于该系统的基因编辑技术在基因工程领域得到广泛应用，极大地加快了该领域研究......
CRISPR/Cas9（Clustered regularly interspaced short palindromic repeats/Cas9）是一个操作便捷、编辑高效和通用性广的基因编辑工......
外源基因在哺乳动物细胞的整合位点会影响宿主细胞的生殖发育,在基因功能研究中常常会先确定其整合的染色体位置.为研究外源基因在......
Within less than a decade since its inception,CRISPR-Cas9-based genome editing has been rapidly advanced to human clinic......
Objective Ghrh is mainly secreted in the hypothalamus,it is difficult to detect its expression in vitro,which has brough......
Precise modifications such as site mutation,codon replacement,insertion or precise targeted deletion are needed for stud......
CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RN
Mouse androgenetic haploid embryonic stem cells (AG-haESCs) can support full-term development of semi-cloned (SC) embryo......
CRISPR/Cas9 和CRISPR/Cpf1 作为当前发展较为成熟的两大基因靶向修饰工具，特别是crRNA 多簇子的设计为多基因靶向修饰提供了可能。......
对生物学功能的精确研究往往需要以可控的方式对基因的功能进行精准干预。而且这些研究也将推动更安全更可控的基因治疗和靶向药物......
规律间隔成簇短回文重复序列及其相关蛋白(clustered regularly interspaced short palindromic repeats-associated,CRISPR/Cas)......
斑马鱼作为一种相对新颖的模式脊椎动物，在生命科学诸多研究领域中已显示出其独特的优势和广阔的前景。然而，与成熟的基因敲除技......
The clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(CRISPR-Cas9) system provides ......
The clustered regularly interspaced short palindromic repeats(CRISPR)-associated nuclease 9(Cas9)-based genome editing t......
CRISPR/Cas基因编辑技术日渐成为基因编辑的主流工具,在酿酒酵母和链霉菌中都有着较为广泛的应用。其中,CRISPR/Cas9技术因其简便......
研究背景Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR associated protein9(CAS9)是一种来自于细......
小麦是我国重要的粮食农作物,小麦产业的发展与粮食安全和社会稳定息息相关,所以提高小麦的产量成为育种工作者的重要目标。TaSPL1......
研究目的:含有三方基序的蛋白11(TRIM11)是E3泛素连接酶家族的成员,在肝癌,肺癌,乳腺癌,宫颈癌等肿瘤中被鉴定为癌基因。但TRIM11......
成簇规律间隔的短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)/Cas9存在于细菌和古细菌中,......
Fusing T5 exonuclease with Cas9 and Cas12a increases the frequency and size of deletion at target si
CRISPR/Cas systems,especially CRISPR/Cas9,generally result in small insertions/deletions,which are unlikely to eliminate......
CRISPR/Cas系统从发现和应用以来,热度持续上升.在过去几年中,这种技术已经成为了基因编辑的标准方案.该技术用一条指导RNA(guide ......
As a robust platform for genome editing,CRISPR/Cas9 is currently being explored for engineering biology or therapeutics,......
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With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced shor......
The clustered regularly interspaced short palindromic repeat (CRISPR) system has emerged as the revolutionary platform f......
Crop breeding aims to generate pure inbred lines with multiple desired traits.Doubled haploid (DH) and genome editing us......
Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgrou
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
养老保险制度是一个非常复杂的课题,我国为规范相关制度,在2014年颁布《企业会计准则第9号——职工薪酬》(CAS 9)。本文通过对CAS ......
As a robust platform for genome editing,CRISPR/Cas9 is currently being explored for engineeringbiology or therapeutics, ......
The waxy gene (Wx) in rice, which encodes the granule bound starch synthase enzyme, is responsible for amylose synthesis......
Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting......
Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice
Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response t......
目的探讨小鼠体外受精（IVF）冻融后用于Cas9注射的可行性。方法新鲜C57BL/6J小鼠卵子IVF后采用冻存管法（EFS20/40）冷冻原核胚（单细胞胚）和......
目的利用CRISPR/Cas9技术敲除巴马小型猪4-羟基苯丙酮酸氧化酶(4-O Hphenylpyruvate dioxygenase,4HPPD/HPD)制备高酪氨酸血症Ⅲ型......
目的探讨CRISPR-Cas9基因编辑技术在乙肝病毒感染治疗中的应用。方法构建CBFβ表达载体，根据从HepG2细胞中提取的RNA，反转得到cDNA，设......
CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the......
目的对CRISPR相关蛋白Cas3、Cas6及Cas9进行表达纯化,并测定其活性。方法在体外构建带有目的基因的质粒,导入大肠杆菌表达系统,通......
