以木薯品种SC124为试材,采用RT-PCR的方法,克隆了木薯可溶性淀粉合成酶基因SSII的全长,并对其进行生物学分析。结果表明:克隆的基因全长包含完整的开放阅读框2 256bp,编码751个氨基酸,通过核苷酸序列同源性分析,该cDNA与基因库中登录的木薯SSII(登录号为:EF667961.1)序列同源性达到99%,但与其它植物的同源性较低,为77%~84%。对木薯SSII多态性分析可知,在木薯基因组内存在等位基因及旁系同源基因。 Using cassava variety SC124 as test material, the full-length cDNA of cassava soluble starch synthase gene SSII was cloned by RT-PCR and its biological analysis was carried out. The results showed that the full-length cDNA of the cloned gene contained 2 256 bp open reading frame (ORF) encoding 751 amino acids. According to the nucleotide sequence homology analysis, the cDNA and the cassava SSII (accession number: EF667961.1 ) Sequence homology reached 99%, but lower homology with other plants, 77% to 84%. The cassava SSII polymorphism analysis shows that there are alleles and paralogs in cassava genome.