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目的:探讨死亡相关蛋白激酶(death-associated protein kinase,DAPK)基因高甲基化与胃癌的发生以及临床病理特征之间的联系。方法:采用甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)法分别检测66例胃癌患者的肿瘤组织、癌旁正常组织、手术前外周血浆以及37例术后血浆中DAPK基因启动子的甲基化状况,以20例健康人的外周血浆和胃镜活检正常胃组织作为对照。结果:胃癌组织中有66.7%(44/66)存在DAPK基因的异常甲基化,显著高于相应的癌旁正常组织[10.6%(7/66)],差异有统计学意义(P<0.001)。术前外周血浆中DAPK基因甲基化阳性率为16.7%(11/66);37例同时有胃癌根治术前后血浆标本的患者中,5例术前血浆甲基化阳性,术后全部转阴。而20例健康人的外周血浆和胃镜活检组织中均未检测到该基因甲基化。结论:DAPK基因在胃癌患者肿瘤组织和外周血浆中的高甲基化可能为胃癌的诊断以及临床预后评估提供有益的线索,手术后血浆中DAPK甲基化状态的变化可能与手术治疗有关。
Objective: To investigate the relationship between the hypermethylation of death-associated protein kinase (DAPK) gene and the occurrence and clinicopathological features of gastric cancer. Methods: The methylation-specific polymerase chain reaction (MSP) was used to detect the activation of DAPK gene in 66 patients with gastric cancer, adjacent normal tissues, preoperative peripheral blood plasma and 37 postoperative plasma Sub-methylation status, peripheral blood plasma and gastroscopic biopsy normal gastric tissue of 20 healthy people as a control. Results: Aberrant methylation of DAPK gene was found in 66.7% (44/66) of gastric cancer tissues, which was significantly higher than that in corresponding adjacent normal tissues [10.6% (7/66)], with significant difference (P <0.001 ). The positive rate of DAPK gene methylation in preoperative peripheral blood was 16.7% (11/66). Among the 37 patients with plasma samples before and after radical resection of gastric cancer, 5 cases had preoperative plasma methylation positive, yin. However, no methylation of this gene was detected in peripheral blood plasma and endoscopic biopsies of 20 healthy people. CONCLUSION: The hypermethylation of DAPK gene in the tumor tissue and peripheral plasma of gastric cancer patients may provide useful clues for the diagnosis of gastric cancer and the evaluation of clinical prognosis. The change of methylation status of DAPK after surgery may be related to the surgical treatment.