论文部分内容阅读
目的探讨死亡相关蛋白激酶(death-associated protein kinase,DAPK)、人类RUNT相关转录因子3(human RUNT-related transcription factor 3,RUNX3)基因启动子区CpG岛在膀胱癌中异常甲基化状态及其在膀胱癌发生中的作用。方法采用实时荧光定量甲基化特异性PCR法检测76例原发性膀胱癌患者的膀胱癌组织标本(膀胱癌组)及39例泌尿系统结石患者的正常膀胱黏膜组织标本(对照组)的DAPK和RUNX3基因启动子区甲基化状态,比较2组DAPK和RUNX3基因启动子区甲基化发生率,分析其甲基化发生率与膀胱癌临床特征间关系。结果膀胱癌组DAPK基因和RUNX3基因启动子区甲基化发生率(64.5%、67.1%)高于对照组(43.6%、38.5%),差异均有统计学意义(P<0.05);膀胱癌组DAPK基因启动子区甲基化发生率在不同分化程度和病理分级上差异均有统计学意义(P<0.05),RUNX3基因启动子区甲基化发生率在肿瘤发生特征、分化程度、病理分级和肿瘤分期上差异均有统计学意义(P<0.05)。结论 DAPK和RUNX3基因启动子区CpG岛高度甲基化可能参与了膀胱癌的发生、发展过程,是膀胱癌组织的遗传学改变之一,对膀胱癌早期诊断、复发监测和预后判断有重要意义。
Objective To investigate the abnormal methylation status of death-associated protein kinase (DAPK), CpG island in promoter region of human RUNT-related transcription factor 3 (RUNX3) gene in bladder cancer In the role of bladder cancer. Methods The real-time fluorescence quantitative methylation-specific PCR method was used to detect the expression of DAPK in bladder mucosa of 76 cases of primary bladder cancer (bladder cancer) and 39 cases of urinary calculi And methylation status of promoter region of RUNX3 gene. The incidence of methylation of promoter region of DAPK and RUNX3 gene in two groups was compared. The relationship between the methylation and the clinical features of bladder cancer was analyzed. Results The incidence of methylation of DAPK gene and RUNX3 gene promoter in bladder cancer group was higher than that in control group (64.5%, 67.1%) (43.6%, 38.5%), the differences were statistically significant (P <0.05) The incidence of methylation of DAPK gene promoter region was significantly different in different degree of differentiation and pathological grade (P <0.05). The incidence of methylation of promoter region of RUNX3 gene was significantly different in tumorigenesis, differentiation, pathology Grading and tumor staging differences were statistically significant (P <0.05). Conclusion The hypermethylation of CpG island in promoter region of DAPK and RUNX3 gene may play an important role in the genesis and progression of bladder cancer, which is one of the genetic changes in bladder cancer. It is of great significance for the early diagnosis, recurrence monitoring and prognosis of bladder cancer .