论文部分内容阅读
目的 :研究肥胖、2型糖尿病、胰岛素抵抗与 β3 AR基因之间的关系及其在家系遗传中的规律。方法 :运用PCR RFLP技术分析了 8个肥胖伴糖尿病家系的 β3 AR基因外显子 64位色氨酸 /精氨酸 (tryptophantoarginineTrp64Arg)多态性。 结果 :β3 AR基因与肥胖进行连锁 ,在常染色体显性遗传模式下 ,单个家系 (家系 3 )最大LOD值为 3 .88510 9(θ =2 0 .0 0 0 0 0 )。将 β3 AR基因与 2型糖尿病进行连锁 ,在常染色体显性遗传模式下 ,单个家系 (家系 2 )LOD最大值为 0 .2 2 2 3 3 6(θ =0 .0 0 0 0 0 0 ) ,在常染色体隐性遗传模式下 ,单个家系(家系 4)LOD最大值为 0 .80 5 0 0 3 (θ =0 .0 0 0 0 0 0 0 )。结论 :Trp64Arg多态性改变是部分家系肥胖产生的直接原因
Objective: To study the relationship between obesity, type 2 diabetes mellitus, insulin resistance and β3 AR gene and its regulation in pedigree. Methods: Polymorphism of exon 64 of tryptophantoarginine (Trp64Arg) in β3 AR gene was analyzed by PCR RFLP in eight obese diabetic families. RESULTS: The β3 AR gene was linked to obesity. In the autosomal dominant inheritance pattern, the maximum LOD of a single pedigree (family 3) was 3.88510 9 (θ = 20.0000). Linking the β3 AR gene to type 2 diabetes mellitus, the maximum LOD of a single pedigree (family 2) was 0.222 3 3 6 (θ = 0. 0 0 0 0 0 0) in an autosomal dominant inheritance pattern In autosomal recessive mode, the maximum LOD of single family (family 4) was 0.80 5 0 0 3 (θ = 0. 0 0 0 0 0 0 0). Conclusion: The change of Trp64Arg polymorphism is the direct cause of obesity in some pedigrees