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目的 探讨β_3-肾上腺素受体(β_3-adr)基因突变与汉族儿童单纯性肥胖、血脂及胰岛素水平关系:分析β_3-adr基因突变与肥胖儿童对膳食干预应答差异的关系。方法 在北京城区4所小学中随机选取311名8～11岁健康儿童,其中超重肥胖儿童73名,非超重儿童243名。所有研究对象均用PCR-RFLP方法检测β_3-adr基因中编码多肽链第64位的色氨酸密码子被精氨酸密码子(Trp64 Arg)取代突变,并进行身体测量及血脂、胰岛素检测,从超重儿童中选出47名单纯性肥胖儿童,男31名,女16名。将其随机分为两组,第一组36人为膳食干预组,第二组11人为对照组。膳食干预3个月再次进行体格测量。结果 73名超重儿童突变频率为0.18,正常儿童突变频率为0.17,两组相比差异无显著性;在修正了年龄、性别后,311名儿童中有基因突变组与无突变组比较,未发现体重指数(BMI)、血压、血脂及空腹胰岛素水平存在差异有显著性;膳食干预研究发现,在膳食干预组无β_3-adr基因Trp64 Arg突变儿童(23名),其体重、BMI增长幅度明显低于非膳食干预组(分别为2.41±0.56对4.43±0.70,P<0.05;0.48±0.24对1.55±0.35,P<0.05),然而携带有β_3-adr基因Trp64 Arg突变儿童(13名)经3个月膳食干预,体重、及BMI增长幅度与非膳食干预对照组相比差异无显著性(分别为4.32±0.69?
Objective To investigate the relationship between β_3-adr (β_3-adr) gene mutation and simple obesity, serum lipids and insulin in Han children: To analyze the relationship between β_3-adr gene mutation and obese children’s response to diet intervention. Methods A total of 311 healthy children aged from 8 to 11 were selected randomly from 4 primary schools in Beijing urban area, including 73 overweight and obese children and 243 non - overweight children. All of the subjects were detected by PCR-RFLP method β_3-adr gene coding polypeptide chain 64th tryptophan codon by arginine codon (Trp64 Arg) substitution mutation, and body measurements and blood lipids, insulin detection, Out of overweight children, 47 simple obese children were selected, 31 males and 16 females. They were randomly divided into two groups, the first group of 36 people for the diet intervention group, the second group of 11 people for the control group. Meal intervention 3 months again physical measurements. Results The frequency of mutation in 73 overweight children was 0.18, and the frequency of mutation in normal children was 0.17. There was no significant difference between the two groups. After adjusting for age and gender, there was no genetic mutation in 311 children with no mutation Body mass index (BMI), blood pressure, blood lipids and fasting insulin levels were significantly different. Dietary intervention study found that children with Trp64 Arg mutation without β_3-adr gene (23) had significantly lower body weight and BMI (2.41 ± 0.56 vs. 4.43 ± 0.70, P <0.05; 0.48 ± 0.24 vs 1.55 ± 0.35, P <0.05, respectively), whereas those with Trp64 Arg mutation in the β_3-adr gene There was no significant difference in the monthly dietary intervention, body weight and BMI between the two groups (4.32 ± 0.69?