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目的:研究小剂量茶碱抗哮喘炎症的可能机制.方法:19名哮喘患者口服茶碱缓释片(200 mg,bid)治疗4周,观察治疗前后症状积分和肺功能变化,并分别采用直接免疫荧光技术、瑞氏染色和ELISA法检测治疗前后高渗盐水诱导痰中CD4~T细胞、嗜酸性粒细胞(Eos)及IL-5和IFN-γ的变化.结果:茶碱治疗可使哮喘患者诱导痰中IL-5水平和Eos数量显著下降(P<0.01),但CD4~T细胞数量和IFN-γ水平无明显变化(P>0.05);患者症状及肺功能明显改善,FEV_(1.0)和FEV_(1.0)％增加(P<0.05).平均血浆茶碱浓度为7.9mg/L(3.9-12.9mg/L).结论:小剂量茶碱通过减少哮喘患者气道IL-5的产生而发挥抗炎作用.
Objective: To investigate the possible mechanism of low-dose theophylline against asthma.Methods: Nineteen asthmatic patients were treated with theophylline sustained-release tablets (200 mg twice daily for 4 weeks), and the symptom scores and lung function changes were observed before and after treatment. Immunofluorescence, Wright staining and ELISA were used to detect the changes of CD4 ~ T cells, eosinophils and IL-5 and IFN-γ in sputum induced by hypertonic saline before and after treatment.Results: (P <0.01), but the number of CD4 ~ T cells and the level of IFN-γ did not change significantly (P> 0.05). The symptoms and lung function of patients were significantly improved. The FEV_ (1.0) ) And FEV 1.0% (P 0.05) .The average plasma theophylline concentration was 7.9 mg / L (3.9-12.9 mg / L) .Conclusion: Low dose of theophylline can reduce the production of IL-5 in the airway of asthmatic patients And play an anti-inflammatory effect.