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采用红光—远红光逆转效应、光温控制及等位性测验等方法，探讨了Ｎ５８ｓ及其转育的光温敏核不育系Ｗ６１５４ｓ，Ｗ７４１５ｓ，３３００１ｓ和３１１１１ｓ的光敏及温敏特性。结果表明，光敏色素参与了Ｎ５８ｓ，３１１１１ｓ和３３００１ｓ育性诱导过程，红光间断长暗期诱导可条件下，其育度显著降低，远红光能部分逆转红光的诱导效应。Ｗ７４１５ｓ育性受光敏色素的调节，Ｗ６１５４ｓ育性基本不受光敏色素调控。Ｗ６１５４ｓ育性在较高温度（＞２７．１２℃）下表现不育，在较低温度下表现可育，诱导完全不育的临界温度在２７．１２℃以上。Ｗ７４１５ｓ，３３００１ｓ，３１１１１ｓ和Ｎ５８ｓ育性主要受光周期调控，温度也存在一定的互作互补效应。参试核不育系Ｎ５８ｓ，３１１１１ｓ，３３００１ｓ与Ｗ７４１５ｓ的光敏核不育基因互为等位，但均与Ｗ６１５４ｓ的核不育基因不等位。因此，利用上述３种方法检测两用核不育水稻的光敏与温敏属性是有效的。
The light-sensitive and thermo-sensitive properties of N58s and their thermo-sensitive genic male sterile line W6154s, W7415s, 33001s and 31111s were studied by red-far red reverse effect, light temperature control and allelopathic test. The results showed that phytochrome participates in the process of fertility induction of N58s, 31111s and 33001s. Under the condition of long-term and dark-duration induction of red light, its fertility is significantly reduced, far red light partially reverses the induction effect of red light. W7415s fertility regulated by phytochromes, W6154s fertility is basically unregulated by phytochromes. Fertility of W6154s was sterile at higher temperature (> 27.12 ℃), fertile at lower temperature, and the critical temperature of induced complete sterility was above 27.12 ℃. The fertility of W7415s, 33001s, 31111s and N58s was mainly regulated by photoperiod, and there was also some interaction and complementarity of temperature. Photoperiod-sensitive genic male sterile lines (N58s, 31111s, 33001s and W7415s) were all allelic to each other, but they were all non-allelic to the genic male sterile line W6154s. Therefore, it is effective to detect the photosensitivity and temperature-sensitive properties of the two genic male sterile rice by the above three methods.