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目的：研究共刺激分子CD80在肝癌细胞中的表达与MHC－I（Majorhistocompatibilitycomplex）分子相互关系，以探讨在肿瘤细胞所诱发的免疫排斥反应中共刺激分子对抗原提呈机制的影响。方法：通过逆转录病毒载体介导，将CD80基因转入肝癌细胞系HHCC，以流式细胞仪检测其表面MHC－I分子的表达情况。同时检测LAK细胞对转染前后的细胞的杀伤效果。结果：表达CD80的肝癌细胞其表面MHC－I分子表达明显增高（P＜0.01），而γ－INF刺激对细胞表面MHG-I分子的表达情况并无影响（P＞0.05）。LAK细胞对转染后细胞的杀伤作用远远大于未转染组（P＜0．01），而γ-INF刺激前后的细胞毒活性几乎无改变（P＞0．05）。结论：转染CD80后肝癌细胞高表达MHC-I分子，增强抗原提呈作用的同时又表达第二信号—共刺激分子CD80，从而可诱发更强的免疫排斥反应。
OBJECTIVE: To investigate the expression of co-stimulatory molecule CD80 in hepatocellular carcinoma cells and the MHC-I (Majorhistocompatibility complex) molecular relationship to investigate the effect of co-stimulatory molecules on antigen presentation mechanism in tumor cell-induced immune rejection. METHODS: CD80 gene was transferred into hepatoma cell line HHCC by retrovirus vector and the expression of MHC-I on the surface was detected by flow cytometry. At the same time, the killing effect of LAK cells on cells before and after transfection was detected. Results: The expression of MHC-I on the surface of CD80-expressing hepatocellular carcinoma cells was significantly increased (P<0.01), while the expression of MHG-I on the cell surface was not affected by γ-INF stimulation (P>0.05). The killing effect of LAK cells on transfected cells was much greater than that of non-transfected cells (P<0.01), while the cytotoxic activity of LAK cells before and after transfection was almost unchanged (P>0.05). Conclusion: After transfected with CD80, hepatoma cells express high levels of MHC-I molecules, enhance the antigen presentation, and at the same time express the second signal-costimulatory molecule CD80, which can induce stronger immune rejection.