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目的研究酵母β-葡聚糖的亚慢性毒性,提出其未观察到有害作用水平(NOAEL)。方法 80只断乳SD大鼠按体重随机分成4组,即对照组和受试物3个剂量组,每组20只,雌雄各半。低、中、高剂量分别为0.62、1.25、2.50 g/kg BW。各剂量组灌胃给予受试物,对照组灌同体积的蒸馏水。动物单笼喂养,自由饮食,每周记录大鼠进食量,每周称2次体重以调整灌胃量,连续观察90 d,于实验中期(45 d)和实验结束采血测各项血液学和临床生化指标,并进行病理组织学检查。结果受试物各剂量组动物体重、进食量、食物利用率、血液学检测指标、临床生化指标、各脏器脏体比与对照组比较,差异均无统计学意义;病理指标也未显示由受试物引起的异常改变。结论根据本次实验结果确定酵母β-葡聚糖NOAEL在雌雄大鼠均为2.50 g/kg BW,即本实验的高剂量,相当于人体推荐量的600倍。
Objective To study the subchronic toxicity of yeast β-glucan and to propose its NOAEL. Methods 80 weaned SD rats were randomly divided into 4 groups according to body weight, namely control group and test group 3 dose groups, 20 in each group, male and female in half. Low, medium and high doses were 0.62,1.25,2.50 g / kg BW. Each dose group was given intragastric test substance, the control group with the same volume of distilled water. The animals were fed in a single cage and free diet. The rats’ food intake was recorded weekly, and the body weight was adjusted twice daily to adjust the gavage volume. The rats were continuously observed for 90 days. Blood samples were collected at the end of the experiment (45 days) Clinical biochemical indicators, and histopathological examination. Results The body weight, food intake, food utilization rate, hematological test indexes, clinical biochemical indexes, viscera and viscera ratio of each dose group were not significantly different from those of the control group, and the pathological indexes did not show any significant difference Abnormal changes caused by the test substance. Conclusions According to the results of this experiment, yeast beta-glucan NOAEL was determined to be 2.50 g / kg BW in both male and female rats, ie high dose in this experiment, equivalent to 600 times the recommended amount of human body.