论文部分内容阅读
目的了解慢性重型乙型病毒性肝炎患者体内HBV的状况。方法选3例慢性重型乙型病毒性肝炎患者,以HBV前S基因为研究区域,采用半巢式PCR法,从此3例患者中血清中扩增出HBV前S基因,并分别将其克隆于噬菌体M13mp18、M13mp19载体中,每例患者随机筛选10个阳性克隆,进行序列分析及遗传距离分析。结果此3例病人存在着“相似株”与“优势株”的混杂及HBVadr与HPBadrwl两种亚型的混合感染。优势株内可能存在引起机体免疫性增高的区域。结论 HBV“相似株”与“优势株”的混杂可能为慢性重型乙型肝炎的一个特点。
Objective To understand the status of HBV in patients with chronic severe hepatitis B virus. Methods Three patients with chronic severe hepatitis B were enrolled in this study. The HBV pre-S gene was used as the research area. The HBV preS gene was amplified from the serum of 3 patients by semi-nested PCR and cloned into Phage M13mp18, M13mp19 vector, each patient randomly selected 10 positive clones for sequence analysis and genetic distance analysis. Results The three patients had a mixture of “similar strains” and “dominant strains” and a mixed infection of two subtypes of HBVadr and HPBadrwl. Advantages of the existence of strains may cause increased body immunity in the region. Conclusion The confounding of HBV “similar strains” and “dominant strains” may be a characteristic of chronic severe hepatitis.