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目的:观察长链非编码RNA(lncRNA)PP7080在胃癌组织和细胞株中的表达，明确其对胃癌细胞MGC803迁移和增殖的影响及分子机制。方法:实时定量聚合酶链反应(Real-time PCR)检测lncRNA PP7080在胃癌组织及癌旁组织、胃癌细胞株及永生化正常胃黏膜上皮细胞株中的表达。选择表达量最少的胃癌细胞株，将lncRNA PP7080的表达质粒和阴性对照质粒分别转染至胃癌细胞，分别命名为lncRNA PP7080组和NC组。Real-time PCR验证转染效果。细胞划痕实验和CCK-8实验检测lncRNA PP7080对胃癌细胞迁移和增殖能力的调控作用。Real-time PCR和Western blot检测转染后细胞中肿瘤转移抑制基因1(n TMSG-1)的表达。采用统计软件SPSS 20.0进行统计分析，正态分布的计量资料以均数±标准差(n Mean±n SD)表示，组间比较采用n t检验。n 结果:lncRNA PP7080在胃癌组织的表达少于癌旁组织[(0.85±0.34)比(5.33±0.76)，n P<0.01]。lncRNA PP7080在胃癌细胞株的表达少于永生化正常胃黏膜上皮细胞(n P<0.05)，在MGC803细胞中表达最少(n P<0.01)。lncRNA PP7080组lncRNA PP7080的表达明显高于NC组，差异有统计学意义(n P<0.01)。NC组和lncRNA PP7080组细胞迁移率分别为(72.67±6.39)%和(26.45±6.63)%，lncRNA PP7080组细胞迁移能力明显降低(n P<0.01)。与NC组相比，lncRNA PP7080组细胞增殖能力明显降低(n P<0.05)。与NC组比较，lncRNA PP7080组MGC803细胞中n TMSG-1的表达明显降低(n P<0.01)。n 结论:lncRNA PP7080在胃癌组织和细胞株中低表达，lncRNA PP7080可通过促进n TMSG-1基因的表达，抑制胃癌细胞MGC803的迁移和增殖能力。n “,”Objective:To observe the expression of long non-coding RNA (lncRNA) PP7080 in gastric cancer tissues and cell lines, and clarify its effect on the migration and proliferation of gastric cancer cell MGC803 and its molecular mechanism.Methods:Real-time PCR was used to detect the expression of lncRNA PP7080 in gastric cancer tissues and adjacent tissues, gastric cancer cell lines and immortalized normal gastric mucosal epithelial cell lines. The gastric cancer cell line with the least expression was selected, and the expression plasmid of lncRNA PP7080 and the negative control plasmid were transfected into gastric cancer cells, respectively, and named as lncRNA PP7080 group and NC group. Real-time PCR to verify the effect of transfection. Cell scratch test and CCK-8 test were used to detect the regulation of lncRNA PP7080 on the migration and proliferation of gastric cancer cells.The statistical saftware SPSS 20.0 was used for statistical analysis, the measurement data of normal distribution were expressed as (n Mean±n SD). Real-time PCR and Western blot were used to detect the expression of tumor metastasis suppressor gene 1 (n TMSG-1) in the transfected cells. The n t test was used for comparison between groups.n Results:The expression of lncRNA PP7080 in gastric cancer tissue was less than that in adjacent tissues [(0.85±0.34) n vs (5.33 ± 0.76), n P<0.01]. The expression of lncRNA PP7080 in gastric cancer cell lines is less than that of immortalized normal gastric mucosal epithelial cells (n P<0.05), and the least expression in MGC803 cells (n P<0.01). The expression of lncRNA PP7080 in the lncRNA PP7080 group was significantly higher than that in the NC group, and the difference was statistically significant (n P<0.01). The cell migration rates of NC group and lncRNA PP7080 group were (72.67±6.39)% and (26.45±6.63)%, respectively, and the cell migration ability of lncRNA PP7080 group was significantly reduced (n P<0.01). Compared with the NC group, the cell proliferation ability of the lncRNA PP7080 group was significantly reduced (n P<0.05). Compared with the NC group, the expression ofn TMSG-1 in MGC803 cells of the lncRNA PP7080 group was significantly reduced (n P<0.01).n Conclusion:lncRNA PP7080 is lowly expressed in gastric cancer tissues and cell lines. lncRNA PP7080 can inhibit the migration and proliferation of gastric cancer cell MGC803 by promoting the expression of TMSG-1 gene.