应用体外培养恶性疟原虫对氯喹敏感株及抗氯喹株测试喹派及羟基喹哌的药效,并以氯喹为对照。原虫培养用Trager和Jensen氏法;药效用体外微量法测定,观察48小时后的减虫率,求得氯喹、喹哌、羟基喹哌对敏感株恶性疟原虫的ED_(50)为81、59及56nM,对抗性株的为563、61及60nM。实验未发现抗氯喹株恶性疟原虫对喹哌及羟基喹哌有明显的交叉抗性。抗氯喹株体外连续培养17天,不加氯喹刺激,抗药性从7倍降至3.4倍。 In vitro culture of Plasmodium falciparum against chloroquine sensitive strains and chloroquine resistant strains of quinpina and hydroxyquipram efficacy, and chloroquine as a control. The protozoal culture was performed by Trager and Jensen’s method. The efficacy was measured by in vitro micro-assay and the worm reduction rate after 48 hours was observed. The results showed that the ED 50 of the sensitive strains of Plasmodium falciparum with chloroquine, quipo, 59 and 56 nM for the resistant strains and 563, 61 and 60 nM for the resistant strains. No chloroquine resistant Plasmodium falciparum was found to have significant cross-resistance to quipo and hydroxyquipronate. Chloroquine strains were cultured continuously for 17 days in vitro without chloroquine stimulation, and the drug resistance was reduced from 7 times to 3.4 times.