论文部分内容阅读
在感染过程中,病原体会表达一系列基因产物以适应体内环境.因此,病原体只在宿主体内表达或高表达的基因可能与致病机制密切相关.本研究用体内诱导抗原技术(in vivo induced antigen technology,IVIAT)来筛选伤寒沙门菌感染过程中体内诱导表达的抗原,共筛选到7个体内诱导(in vivo induced,IVI)抗原,分别是BcfD(菌毛结构亚单位)、GrxC(谷氧还蛋白3)、SapB(ABC转运系统)、T3663(ABC未知转运系统)、T3816(可能的有关硫氰酸酶的硫转移酶)、T1497(可能的TonB依赖受体)和T3689(功能未知),其中BcfD,GrxC,SapB,T3663和T3689这5个抗原与吸附处理后的健康志愿者的血清无交叉免疫反应.这些筛选到的体内诱导抗原是新药和疫苗开发的潜在靶标,同时也有利于新诊断方法的研究.
During the process of infection, the pathogen will express a series of gene products to adapt to the environment. Therefore, the genes that the pathogen only express in the host or highly expressed may be closely related to the pathogenic mechanism.In this study, in vivo induced antigen technology, IVIAT) to screen for in vivo induced antigens during Salmonella typhi infection. Seven in vivo induced anti-IVI antigens were selected and identified as BcfD (pilus structural subunit), GrxC Protein 3), SapB (ABC transport system), T3663 (ABC unknown transport system), T3816 (possibly sulfurase transferase), T1497 (possible TonB dependent receptor) and T3689 (unknown function) Among them, the five antigens of BcfD, GrxC, SapB, T3663 and T3689 did not cross-react with the sera of healthy volunteers after adsorption treatment.These selected in vivo induced antigens were potential targets for the development of new drugs and vaccines, Study of diagnostic methods.