CUTINASE相关论文
以基础培养基(查彼培养基+碳酸钙+苹果角质)为营养优化了长柄链格孢(Alternaria longipes)产角质酶的环境和营养条件.结果表明,最......
本文对烟草赤星病菌Alternuria longipes产生的角质酶进行了纯化及性质测定.在含有苹果角质(诱导物)的液体培养基中培养A.longipes......
本试验旨在通过角质降解菌株X8P的种属鉴定、发酵条件优化和酶学性质研究,探讨降解植物表面角质层,进一步改善动物对植物纤维利用......
应用大肠杆菌（Escherichia coli,E.coli）BL21（DE3）表达系统实现特异腐质霉（Humicola insolens,H.insolens）来源角质酶的重组表达。对重组......
采用PCR法克隆了桃褐腐病菌的角质酶基因cut1,构建了诱导型表达载体pET28a-cut1,转化大肠杆菌E.coli（BL21）,获得重组菌pET28a-cut1/B......
克隆来自Thermob乒dafttsca（嗜热放线菌）的编码角质酶基因咖，并在N端人为添加了α—factor信号肽，以引导重组角质酶的分泌表达。在成功......
研究了环境和营养条件等对嗜热放线菌Thermobifida fusca WSH03-11生长及产角质酶的影响;并考察了苹果角质对角质酶诱导效果,分析......
为进一步提高重组Bacillus subtilis WSHB06-07发酵生产角质酶的产量和生产强度,在pH两阶段控制策略的基础上,考察了温度（27～40℃）对......
通过跟踪发酵液中pNPB水解酶活性,对角质诱导的Thermobifida fusca发酵液进行分离纯化.采用活性炭脱色、硫铵沉淀、Phenyl HP疏水......
以放线菌Thermobifida fusca WSH03—11——高产角质酶突变株为出发菌株，在摇瓶中考察了碳、氮源等条件以及接种量、装液量和初始pH......
研究了角质酶W5的特性及脱墨浆浓度、碎浆时间及酶用量对角质酶W5应用于混合办公废纸(MOW)脱墨效果的影响。结果表明:所选3个因素......
Thermostability of two homologous cutinases, Cut1 and Cut2 from Thermobifida fusca NRRL B-8184 was inves-tigated at comb......
Thermostability of two homologous cutinases, Cut1 and Cut2 from Thermobifida fusca NRRL B-8184 was inves-tigated at comb......
Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepa
The development of medium for the production of cutinase from Pseudomonas cepacia NRRL B 2320 was carried out using Plac......
Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepa
The development of medium for the production of cutinase from Pseudomonas cepacia NRRL B 2320 was carried out using Plac......
研究了乙醇溶液对角质酶性质的影响,同时用圆二色光谱和荧光光谱法对经过硫酸铵沉淀、镍柱亲和层析等方法获得的电泳纯角质酶进行......
Cutinases are hydrolytic enzymes used by phytopathogenic fungi to gain entry into plants by breaking down the cuticular ......
A double mutantEst1, which is a plastic degrading cutinase-type esterase in Thermobifida alba, has been over-expressed i......
