Cellulose tris(4 methylphenylcarbamate), amylose tris(3,5 di methylphenylcarbamate) and amylose tris(phenylcarbamate) were prepared by the method reported by Okamoto and were coated onto an aminopropylated mesoporous spherical silica gel. These final products were used as chiral stationary phases of high performance liquid chromatography for the eighteen structurally related biphenyl compounds. The resolution was made using normal phase methodology with a mobile phase consisting of n hexane alcohol (ethanol, 1 propanol, 2 pro panol or 1 butanol). The effects of various aliphatic alcohols in the mobile phase were studied. The structural features of the solutes that influence their k ′were discussed. A dominant effect of trifluoroacetic acid on chiral separation of acidic solutes was noted. Cellulose tris (4 methylphenylcarbamate), amylose tris (3,5 di methylphenylcarbamate) and amylose tris (phenylcarbamate) were prepared by the method reported by Okamoto and were coated onto an aminopropylated mesoporous spherical silica gel. These final products were used as chiral stationary phases of high performance liquid chromatography for the eighteen structurally related biphenyl compounds. The resolution was made using normal phase methodology with a mobile phase consisting of n hexane alcohol (ethanol, 1 propanol, 2 pro panol or 1 butanol). The effects of various aliphatic alcohols in the mobile phase were studied. The structural features of the solutes that influence their k ’were discussed. A dominant effect of trifluoroacetic acid on chiral separation of acidic solutes was noted.