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目的:本研究拟探讨桥粒芯胶蛋白-1与头颈部鳞状细胞癌发生及预后的相关性。方法:本研究通过对NCBI数据库GEO获取的基因芯片数据进行统计学分析,并且采用头颈部鳞状细胞癌组织芯片予以验证。免疫组织化学的方法检测了组织芯片中芯胶蛋白-1的表达,并采用统计学方法分析了芯胶蛋白-1的表达与头颈部鳞状细胞癌发生、分化、预后等的相关性。结果:对GEO获取的芯片数据分析发现芯胶蛋白-1在头颈部鳞状细胞癌中表达增高,且表达增高的患者预后较差。组织芯片的验证实验显示结果与GEO数据库获取数据分析结果基本一致,在头颈部鳞状细胞癌中DSC1的表达显著高于正常组织,且在低分化头颈部鳞状细胞癌中表达高于高分化鳞状细胞癌。结论:桥粒芯胶蛋白-1的增高可促进头颈部鳞状细胞癌的发生,且与肿瘤分化相关。此外,其表达增高提示患者预后不良。
Objective: This study was to investigate the relationship between desmocollin-1 and the occurrence and prognosis of head and neck squamous cell carcinoma. Methods: We analyzed the gene chip data obtained from the GEO NCBI database and validated the head and neck squamous cell carcinoma tissue microarray. Immunohistochemistry was used to detect the expression of Corein-1 in tissue microarray. The correlation between expression of Corein-1 and occurrence, differentiation and prognosis of squamous cell carcinoma of head and neck was analyzed by statistical methods. RESULTS: Cholesterol-1 expression in GEO-derived chip data showed increased expression in head and neck squamous cell carcinomas and poor prognosis in patients with elevated expression. Tissue chip validation experiments showed that the results and the GEO database access to data analysis results are basically the same, in the head and neck squamous cell carcinoma DSC1 expression was significantly higher than normal tissue, and poorly differentiated head and neck squamous cell carcinoma was higher than Well-differentiated squamous cell carcinoma. Conclusion: Increased desmocollin-1 can promote the occurrence of squamous cell carcinoma of the head and neck, which is related to tumor differentiation. In addition, its increased expression prompts patients with poor prognosis.