论文部分内容阅读
目的 观察支气管哮喘不同状态时外周淋巴细胞肾上腺素能受体 (βAR)及β2 ARmRNA水平的变化。方法 用放射配基结合受体分析法 ,放射免疫法 ,逆转录聚合酶链反应 (PT PCR)及原位杂交等方法测定哮喘患者不同状态下 β2 AR密度 ,β2 ARmRNA水平 ,血浆cAMP和cGMP水平。结果 缓解期哮喘患者外周淋巴细胞 βAR的密度较正常人明显升高 ,血浆cAMP水平无显著差异 ,而发作期哮喘患者βAR及血浆cAMP水平均较正常人明显降低 ;缓解期哮喘患者外周淋巴细胞β2 ARmRNA水平与正常人无显著差异。结论 βAR数量、功能及其信使核糖核酸水平与哮喘不同发病状态有关 ,其改变仅为哮喘病程中伴随出现的变化 ,不是其发病的原发因素。
Objective To observe the changes of peripheral blood adrenergic receptor (βAR) and β2 AR mRNA in different states of bronchial asthma. Methods Radioimmunoassay, radioimmunoassay, PT PCR and in situ hybridization were used to determine the levels of β2 AR, β2 AR mRNA, plasma cAMP and cGMP in different states of asthma . Results The density of βAR in peripheral lymphocytes in asthmatic patients was significantly higher than that in normal controls and the level of plasma cAMP was not significantly different in asthmatic patients. The levels of βAR and cAMP in patients with asthma were significantly lower than those in normal controls There was no significant difference in ARmRNA level between the two groups. Conclusions The quantity and function of βAR and the level of messenger ribonucleic acid are related to the different pathogenesis of asthma. The changes of βAR are only the accompanying changes in the course of asthma, but not the primary factors of its onset.