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目的探讨氧自由基对支气管哮喘患者 β 肾上腺素受体 (β AR)的影响。方法采用放射配基受体结合分析法、放免法和分光光度法测定 2 8例哮喘患者和正常对照组 12例外周血淋巴细胞 β AR、超氧化物歧化酶 (SOD)、丙二醛(MDA)及环磷酸腺苷 (cAMP)含量。结果哮喘组较对照组SOD含量明显降低 (P <0 .0 1) ,而MDA水平明显增加 (P<0 .0 1) ;哮喘组较对照组 β AR数量明显减少(P <0 .0 1) ,cAMP降低 (P <0 .0 5 )。哮喘组外周血SOD含量与β AR呈显著正相关 (r =0 .4 1,P <0 .0 1) ,与cAMP显著正相关 (r =0 .32 ,P <0 .0 1)。MDA与 β AR呈显著负相关 (r =- 0 .2 8,P <0 .0 1) ,与cAMP显著负相关 (r =- 0 .2 5 ,P <0 .0 1)。结论氧自由基增多是 β AR功能低下的一个因素。β 受体功能低下并非为哮喘患者所固有 ,是哮喘发作肺部非特异性炎症后产生的继发性改变。
Objective To investigate the effects of oxygen free radicals on β-adrenergic receptor (β AR) in patients with bronchial asthma. Methods Radioligand receptor binding assay, radioimmunoassay and spectrophotometry were used to detect the levels of β AR, superoxide dismutase (SOD) and malondialdehyde (MDA) in peripheral blood lymphocytes in 28 asthmatic patients and 12 healthy controls. ) And cyclic adenosine monophosphate (cAMP) content. Results Compared with the control group, the content of SOD in the asthma group was significantly decreased (P <0.01) and the MDA level was significantly increased (P <0.01). The number of β AR in the asthma group was significantly lower than that in the control group (P <0.01) ), cAMP decreased (P <0.05). The level of SOD in asthmatic group was positively correlated with β AR (r = 0.41, P <0.01), and positively correlated with cAMP (r = 0.32, P <0.01). There was a significant negative correlation between MDA and β AR (r = - 0.28, P <0.01) and negatively correlated with cAMP (r = - 0.25, P <0.01). Conclusion The increase of oxygen free radicals is a factor of the low function of β AR. Beta-receptor dysfunction is not inherent in patients with asthma and is a secondary alteration of the lung’s unspecific inflammation after asthma attacks.