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目的研究脱氢野百合碱(DHMC)诱导犬肺动脉高压模型的合适剂量,以提高犬肺动脉高压建模成功率。方法将28只犬随机分成3组,即3mg·kg~(-1) DHMC组(n=12)、2 mg·kg~(-1) DHMC组(n=8)和1.5 mg·kg~(-1) DHMC组(n=8)。所有犬于右心导管测定各组血流动力学参数后,右心注射不同剂量的DHMC,观察8周。记录观察期间每组犬的死亡只数,并行肺组织病理切片检查。结果 3 mg·kg~(-1) DHMC组导致动物死亡率高达58.33%;而2 mg·kg~(-1)组注射DHMC诱导犬肺动脉高压模型的成功率为100%,动物死亡率为0;1.5 mg·kg~(-1) DHMC组肺动脉压力未见明显升高。结论野百合碱对于肺血管毒性损伤作用呈剂量相关性,减少DHMC注射剂量,以2 mg·kg~(-1)注射,不仅犬存活率较高,而且能成功诱导形成肺动脉高压模型。
Objective To study the appropriate dose of DHPP induced model of pulmonary hypertension in dogs and to improve the success rate of modeling pulmonary hypertension in dogs. Methods 28 dogs were randomly divided into 3 groups: DHMC group (n = 12), DHMC group (n = 8) and 1.5 mg · kg ~ (-1) -1) DHMC group (n = 8). All dogs in the right heart catheterization measured hemodynamic parameters of each group, the right injection of different doses of DHMC, observed for 8 weeks. Record the number of deaths of dogs in each group during the observation period, parallel pathological examination of lung tissue. Results The mortality of DHMC group was as high as 58.33% at 3 mg · kg -1, while that of 2 mg · kg -1 group was 100% with DHMC injection. The mortality rate of animals was 0 ; 1.5 mg · kg -1 DHMC group pulmonary artery pressure was no significant increase. Conclusion The effect of monocrotaline on pulmonary vascular injury was dose-dependent. Decreasing DHMC dose and injecting 2 mg · kg -1 could not only increase the survival rate of the dog, but also induce the formation of pulmonary hypertension.