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将自体富集骨髓间充质干细胞(MSCs),快速复合多孔β磷酸三钙(β-TCP),构建生物活性复合材料(MSCs/β-TCP)。通过分层随机分组对照比较了MSCs/β-TCP与单纯β-TCP及自体骨在羊腰椎后路融合的效果。测定富集后碱性磷酸酶染色为阳性的细胞集落单位(CFUs/ALP+,即MSCs数量)变化情况。用扫描电镜观察了MSCs与β-TCP短期复合情况。通过X线、CT扫描和组织学观察对比植入材料成骨情况及转归。结果表明,富集后MSCs数量增加约3.9倍,短期复合2 h后MSCs即可在多孔β-TCP内壁黏附;X线检查,MSCs/β-TCP组与自体骨组的融合区逐渐有中高密度影形成,CT扫描测定MSCs/β-TCP组与自体骨组的融合率,分别为92%和100%,明显高于单纯-βTCP组(58%)。硬组织形态计量学证实,复合材料MSCs/-βTCP与自体骨的骨再生能力相当,优于单纯β-TCP材料。
MSCs / β-TCP will be enriched by autologous enrichment of bone marrow mesenchymal stem cells (MSCs), rapid assembly of porous β-tricalcium phosphate (β-TCP) and bioactive composite materials. The effect of MSCs / β-TCP fusion with pure β-TCP and autologous bone on the posterior approach of the lumbar spine was compared by stratified randomized controlled trials. The concentration of alkaline phosphatase-positive colony units (CFUs / ALP +, the number of MSCs) after the enrichment was measured. Scanning electron microscopy of MSCs and β-TCP short-term complex situation. The X-ray, CT scan and histological observation were used to compare the osteogenesis and prognosis of the implanted materials. The results showed that the number of MSCs increased by about 3.9-fold after MSCs enrichment, and MSCs could adhere to the porous β-TCP wall after short-term complexation for 2 h. X-ray examination, MSCs / β-TCP group and autologous bone group gradually showed medium- The fusion rate of MSCs / β-TCP group and autologous bone group was 92% and 100%, respectively, which was significantly higher than that of pure -βTCP group (58%). Hard tissue histomorphometry confirmed that the bone regeneration capacity of composite MSCs / -βTCP was significantly higher than that of autologous bone, which was better than pure β-TCP material.