论文部分内容阅读
目的 研究大肠癌中nm 2 3、CD44基因的表达及其在大肠癌局部进展中的作用。方法 用FCM (流式细胞术 )方法 ,测定 6 4例大肠癌患者nm 2 3和CD44基因蛋白。结果 蛋白表达量以荧光指数 (flurescentindex)表达 ,大肠癌nm2 3(FI =0 .84± 0 .0 9) ,正常粘膜 (FI =1.0± 0 .0 3) ,P <0 .0 0 1。大肠癌CD44(FI =1.43± 0 .14 ) ,正常粘膜 (FI =1.0± 0 .33) ,P <0 .0 0 1。大肠癌增殖指数 (PI =2 8.6 0± 8.0 7) ,正常粘膜 (PI =19.6 1± 6 .94) ,P <0 .0 2。Duke′s分期C +D :nm 2 3(FI =0 .80± 0 .0 8)CD44(FI =1.46± 0 .13) ;Duke′s分期A +B :nm2 3(FI =0 .89± 0 .10 )、CD44(FI =1.34± 0 .13) ,P均 <0 .0 5。浸润和溃疡型癌肿 :nm2 3(FI =0 .80± 0 .0 8) ,CD44(FI =1.48± 0 .12 ) ;隆起型 :nm 2 3(FI =0 .88± 0 .10 )、CD44(FI =1.35± 0 .13) ,P均 <0 .0 5。异倍体癌 :CD44(FI =1.45± 0 .15 ) ;二倍体癌 :CD44(FI =1.33± 0 .11) ,P <0 .0 5。结论 nm 2 3基因蛋白在大肠癌形成、浸润、转移中有负调控作用 ,CD44基因蛋白起正调控作用 ;nm2 3基因蛋白低表达和CD44基因蛋白高表达的大肠癌预后差。
Objective To study the expression of nm 2 3 and CD44 in colorectal carcinoma and its role in the local progression of colorectal carcinoma. Methods The nm 2 3 and CD44 genes of 64 patients with colorectal cancer were determined by FCM (Flow Cytometry). Results The protein expression levels were expressed as flurescentindex. Colorectal cancer nm23 (FI = 0.84 ± 0.09), normal mucosa (FI = 1.0 ± 0.30), P <0.01. Colorectal cancer CD44 (FI = 1.43 ± 0.14), normal mucosa (FI = 1.0 ± 0.33), P <0.001. Proliferation index of colorectal cancer (PI = 22.68 ± 8.07), normal mucosa (PI = 19.61 ± 6.94), P <0. Duke’s stage C + D: nm 2 3 (FI = 0.80 ± 0.08) CD44 (FI = 1.46 ± 0.13); Duke’s stage A + B: nm2 3 ± 0.10), CD44 (FI = 1.34 ± 0.13), all P <0.05. Infiltrative and ulcerative carcinomas: nm2 3 (FI = 0.80 ± 0.08), CD44 (FI = 1.48 ± 0.12), bulge type: nm 2 3 (FI = 0.88 ± 0.10) , CD44 (FI = 1.35 ± 0.13), P <0.05. Aneuploid carcinoma: CD44 (FI = 1.45 ± 0.15); diploid carcinoma: CD44 (FI = 1.33 ± 0.11), P <0.05. Conclusion The nm23 protein has a negative regulatory effect on the formation, invasion and metastasis of colorectal cancer, and the CD44 gene protein plays a positive regulatory role. The poor prognosis of colorectal cancer with low expression of nm23 gene and high expression of CD44 gene is significant.