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[目的]研究黄芩苷对高糖诱导的人脐静脉血管内皮细胞株(CRL1730)细胞凋亡及细胞增殖抑制的影响。[方法]用不同浓度黄芩苷(0.1、0.2、0.4g/L)、D-葡萄糖(5.5、15、25、35、45、55mmol/L)共同作用培养的人脐静脉血管内皮细胞株(CRL1730)48h,流式细胞仪(flow eytometry,FCM)测定内皮细胞的凋亡率、细胞周期,噻唑蓝法(MTT法)测增殖率,酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测可溶性细胞间黏附分子-1(Serum soluble intercellular adhesion molecule-1,sICAM-1)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α),化学法检测一氧化氮(Nitric oxide,NO)水平,黄嘌呤氧化酶法检测超氧化物歧化酶(SOD)含量,并与正常糖浓度(5.5mmol/L)培养基组比较。[结果]正常糖浓度时,除MTT外,黄芩苷各浓度下指标无统计学差异。在使用高糖培养基时黄芩苷作用组凋亡率下降,G2M期细胞比例、MTT值明显高于单纯高糖组(P﹤0.05),并同时降低sICAM-1、TNF-α,使NO含量增加、SOD活力增高。[结论]黄芩苷对高糖导致的CRL1730细胞凋亡率的增加、细胞周期改变、增殖能力下降具有保护作用。其原因可能与其抑制炎症反应、氧化应激等因素有关。
[Objective] To investigate the effects of baicalin on apoptosis and proliferation inhibition of human umbilical vein endothelial cell line (CRL1730) induced by high glucose. [Method] The human umbilical vein endothelial cell line (CRL1730) cultured with different concentrations of baicalin (0.1,0.2,0.4g / L) and D-glucose (5.5,15,25,35,45,55mmol / L) ) 48h. Flow cytometry (FCM) was used to detect the apoptosis rate of endothelial cells, cell cycle, MTT proliferation rate and enzyme-linked immunosorbent assay (ELISA) Serum soluble intercellular adhesion molecule-1 (sICAM-1) and tumor necrosis factor-α (TNF-α) were detected by chemical method. Nitric oxide (NO) Level, the content of superoxide dismutase (SOD) was detected by xanthine oxidase method and compared with the normal sugar concentration (5.5 mmol / L) medium group. [Result] There was no significant difference in the concentration of baicalin except normal MTT when the normal sugar concentration was reached. When using high glucose medium, the apoptosis rate of baicalin group decreased, the cell ratio and MTT value in G2M phase were significantly higher than those in high glucose group (P <0.05), and the sICAM-1 and TNF- Increase, increased SOD activity. [Conclusion] Baicalin has a protective effect on the apoptosis of CRL1730 cells induced by high glucose, cell cycle and the decrease of proliferative capacity. The reason may be related to its inhibition of inflammatory reactions, oxidative stress and other factors.