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为了快速、准确地筛选大豆耐菌核病候选资源,采用茎中可溶性色素水平测定法,评价了150份部分核心种质和国外种质及100份近15年东北育成品种的菌核病耐病性,并通过草酸浸根产生的植株萎蔫反应,验证了鉴定结果的准确性;同时,分析了花色(茎色)、叶形、生育期与耐病性的相关性。聚类分析将资源耐病性分为5个级别,分别是高耐(3.20%)、耐(7.60%)、中感(21.60%)、感病(46.00%)和高感(21.60%),并筛选出27份高耐病和耐病的候选资源,其中种质材料中具有相对较多的耐病性资源。草酸浸根植株萎蔫反应与茎中可溶性色素评价结果相符。耐病强度与农艺性状相关分析表明:叶形和生育期与耐病性不相关,但花色(茎色)与耐病性显著相关。可见,在反映草酸致病机制方面,茎中可溶性色素水平测定法能够进行大豆资源菌核病耐病性鉴定和评价;在遗传多态性丰富的大豆资源中可能会筛选到更多的耐菌核病的资源,可能多以紫花为主。
In order to quickly and accurately screen soybean candidate candidate for resistance to sclerotinia sclerotiorum, the susceptibility to sclerotinia of 150 partial core collections, 100 foreign germplasms, and 100 northeast bred cultivars were evaluated using the soluble pigment assay in stems The wilting reaction of oxalic acid was used to verify the accuracy of the identification results. The correlation between flower color (stem color), leaf shape, growth period and disease resistance was also analyzed. Cluster analysis divided the disease resistance into five levels: high resistance (3.20%), resistance (7.60%), moderate infection (21.60%), susceptible disease (46.00%) and high susceptibility A total of 27 high-disease and disease-resistant candidate resources were screened out, among which germplasm materials had relatively more disease-resistant resources. The wilting response of oxalate dipped roots was consistent with the results of soluble pigment evaluation in stems. Correlation analysis of disease resistance and agronomic traits showed that leaf shape and growth stage were not related to disease resistance, but flower color (stem color) was significantly correlated with disease resistance. It can be seen that in the pathogenesis of oxalic acid, the soluble pigment level in stems can be used to identify and evaluate the disease resistance of soybean sclerotinia. More resistant bacterial nuclei may be screened in soybean germplasm rich in genetic polymorphism Disease resources, may be more purple flowers.