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综合微流控技术与激光技术,对流体环境中细胞在激光作用下的运动情况进行了受力分析,并对其作用机理进行了研究。首先针对不同物理尺寸的细胞在侧向激光作用下的运动轨迹进行仿真,并根据仿真结果来优化设计芯片的微通道结构,提高其分离效率。同时在制作微流控芯片的过程中,将光纤直接固定在模板上用PDMS进行浇注,这样得到的芯片可以直接将激光引入,形成侧向激光对通道内细胞进行干预,并使其运动轨迹发生改变。实验结果表明,在鞘流和细胞液分别以0.2μL/min和0.1μL/min的速度进样时,使用960 nm,200 mW的泵浦激光可以使细胞轨迹发生偏移。实验结果与仿真结果基本相符。
Based on microfluidic technology and laser technology, the force analysis of the movement of cells under the action of laser in fluid environment was carried out and the mechanism of action was studied. Firstly, the motion trajectories of cells with different physical size under the lateral laser are simulated, and the microchannel structure of the chip is optimized according to the simulation results to improve the separation efficiency. At the same time in the process of making microfluidic chip, the fiber is directly fixed on the template with PDMS for casting, so that the chip can be directly introduced into the laser, the formation of lateral laser intervening cells in the channel, and make its trajectory change. Experimental results show that when the sheath flow and the cytosol are injected at 0.2 μL / min and 0.1 μL / min respectively, the cell trajectories can be shifted by using a pump laser of 960 nm and 200 mW. The experimental results are in good agreement with the simulation results.