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目的:研究表皮生长因子(EGF)对体外培养的大鼠牙囊细胞增殖活性的影响。方法:原代培养Wistar大鼠牙囊细胞,选择生长良好的第3代细胞,分别加入不同浓度的EGF(0.5、1、5、10、50 ng/mL)与牙囊细胞共孵育5 d,MTT法对比观察不同浓度的EGF对牙囊细胞增殖活性的影响。数据采用SPSS 13.0软件包进行方差分析。结果:在EGF浓度为5～10 ng/mL时,牙囊细胞的增殖活性显著增高(P<0.05),10 ng/mL时达到最高(P<0.01)。结论:EGF可作用于牙囊细胞,合适浓度的EGF能显著增加牙囊细胞的增殖活性。
Objective: To study the effect of epidermal growth factor (EGF) on proliferation of rat dental follicle cells cultured in vitro. Methods: The primary cultured Wistar rat dental follicle cells were selected and the third generation of well-growing cells were selected and incubated with different concentrations of EGF (0.5, 1, 5, 10, 50 ng / mL) for 5 days respectively. The effect of different concentrations of EGF on proliferation of dental follicle cells was observed by MTT assay. Data were analyzed using SPSS 13.0 software package. Results: The proliferative activity of dental follicles was significantly increased (P <0.05) at EGF concentration of 5 ~ 10 ng / mL, and reached the peak at 10 ng / mL (P <0.01). CONCLUSION: EGF can act on dental follicle cells. The suitable concentration of EGF can significantly increase the proliferation activity of dental follicle cells.