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应用抗猪囊虫抗原的单克隆抗体反向间接血凝试验(IHAT),对临床确诊的囊虫病患者进行了血清循环抗原(CAg)的检测,阳性率为51.14%(67/131)。对19例脑型囊虫病患者同时测定了血清和脑脊液中的CAg,阳性率分别为47.36%和78.94%,总阳性率为84.21%。对治疗0.53后的19例患者检测时,血清和脑脊液中CAg均呈阴性反应,而检测抗体仍呈阳性反应。50例正常人、49例其它寄生虫病及5例其它脑部疾病患者脑脊液的CAg均呈阴性反应。结果提示:测定CAg对囊虫病活动性感染的诊断及疗效判定均具有一定的实用价值。
The monoclonal antibody reverse indirect hemagglutination test (IHAT) of anti-swine cysticercosis antigen was used to detect serum circulating antigen (CAg) in patients with clinically confirmed cysticercosis. The positive rate was 51.14% (67/131 ). Serum and cerebrospinal fluid levels of CAg were measured simultaneously in 19 patients with cerebral cysticercosis. The positive rates were 47.36% and 78.94% respectively, with a total positive rate of 84.21%. The detection of 19 patients after treatment of 0.53, serum and cerebrospinal fluid CAg were negative, and the test antibody was still positive. The plasma levels of CAg in 50 normal subjects, 49 other parasitic diseases and 5 patients with other brain diseases were all negative. The results suggest that the determination of CAg in the diagnosis and treatment of active infection of cysticercosis have a certain practical value.