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背景:作者前期研究证实心房钠尿肽和5-羟色胺共同存在于胃肠嗜铬细胞的同一分泌颗粒中,那么心房钠尿肽对5-羟色胺的合成与分泌是促进还是抑制,目前仍处于研究阶段。目的:通过模拟胃黏膜内分泌和腔分泌心房钠尿肽来证实对胃肠嗜铬细胞中5-羟色胺合成与分泌的影响。设计:随机对照动物实验。单位:承德医学院免疫学实验室。材料:实验于2004-10/2007-07在省级重点实验室承德医学院免疫学实验室完成。选用40只雄性成年Wistar大鼠,由承德医学院实验动物科提供,实验过程中对动物的处置符合动物伦理学标准。实验用心房钠尿肽、5-羟色胺抗体为美国Santa Cruz Biotechnology公司产品。方法:随机将40只大鼠均分至内分泌和外分泌2个平行实验中,各分为对照组10只和实验组10只。通过胃腔直接注射心房钠尿肽(28μg14mg/L)模拟外分泌产生的心房钠尿肽,通过舌下静脉注射心房钠尿肽14μg,14mg/L)模拟内分泌产生的心房钠尿肽;并以免疫组织化学,透射电镜的超微结构的测量学的方法和高压液相色谱电化学检测的方法对心房钠尿肽刺激后胃内5-羟色胺免疫反应细胞和内分泌颗粒的数量及血清中的5-羟色胺含量,与注射生理盐水的对照组进行对比。主要观察指标:模拟胃黏膜内分泌和腔分泌心房钠尿肽对胃内5-羟色胺免疫反应阳性细胞密度、分泌颗粒的数密度及血清中的5-羟色胺的影响。结果:模拟心房钠尿肽外分泌和内分泌对大鼠胃内5-羟色胺分泌的影响:与对照组相比,胃内5-羟色胺免疫反应阳性细胞密度及分泌颗粒的数密度显著增多(P<0.05),血清中的5-羟色胺含量显著减少(P<0.05)。结论:外分泌和内分泌的心房钠尿肽对5-羟色胺的释放均有明显的抑制作用。
BACKGROUND: Previous studies have shown that atrial natriuretic peptide and serotonin coexist in the same secretory granules of gastrointestinal pheochromocytoma. So, whether atrial natriuretic peptide promotes or inhibits the synthesis and secretion of serotonin is still under study stage. OBJECTIVE: To confirm the effects of atrial natriuretic peptide (PAA) on the synthesis and secretion of serotonin in gastrointestinal chromaffin cells by simulating the gastric mucosal endocrine and cavity secretions. Design: Randomized controlled animal experiments. Unit: Chengde Medical College Immunology Laboratory. Materials: The experiment was performed at Immunology Laboratory, Chengde Medical College, Provincial Key Laboratory from October 2004 to July 2007. Forty male adult Wistar rats were selected and provided by Experimental Animal Branch of Chengde Medical College. During the experiment, animals were treated according to animal ethical standards. Experimental natriuretic peptide, serotonin antibody for the United States Santa Cruz Biotechnology company. Methods: Forty rats were randomly divided into two parallel experiments of endocrine and exocrine, which were divided into control group (n = 10) and experimental group (n = 10). The atrial natriuretic peptide produced by exocrine was injected directly into the gastric cavity through atrial natriuretic peptide (28μg14mg / L), and the atrial natriuretic peptide produced by endocrine was simulated by intravenous injection of atrial natriuretic peptide (14μg, 14mg / L) through the sublingual vein. Histochemistry, transmission electron microscopy ultrastructure of the measurement method and high pressure liquid chromatography electrochemical detection method of atrial natriuretic peptide after stimulation of gastric serotonergic immune response cells and endocrine particles and the amount of 5- Serotonin content, compared with the control group injected with saline. MAIN OUTCOME MEASURES: The effects of gastric mucosal endocrine and cavity-secreting atrial natriuretic peptide on serotonin-immunoreactive positive cell density, number density of secretory granules and serotonin in serum were simulated. Results: The effects of atrial natriuretic peptide exocrine and endocrine on gastric serotonin secretion in rats: Compared with the control group, the number of serotonin-immunoreactive positive cells and the number density of secretory granules in the stomach increased significantly (P <0.05 ), Serum serotonin levels were significantly reduced (P <0.05). Conclusion: The exocrine and endocrine atrial natriuretic peptides have a significant inhibitory effect on the release of 5-hydroxytryptamine.