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目的 研究肠易激综合征 (IBS)患者的肠嗜铬(EC)细胞及其合成和储存 5 HT的功能变化。方法 按罗马Ⅱ诊断标准 ,并依其分型标准区分腹泻型及便秘型IBS。取IBS患者肠镜活检标本进行Envision法免疫组化染色。部分活检标本电镜下观察EC细胞形态及功能。免疫组化染色及电镜观察均
Objective To investigate the functional changes of enterochromaffin (EC) cells in patients with irritable bowel syndrome (IBS) and their synthesis and storage of 5 HT. Methods According to Rome Ⅱ diagnostic criteria, and according to their classification criteria to distinguish between diarrhea and constipation type IBS. Enteroscopy biopsy specimens from patients with IBS Envision immunohistochemical staining. The morphology and function of EC cells were observed by electron microscopy in some biopsy specimens. Immunohistochemical staining and electron microscopy were