研究结果表明:(1)甘蓝型油菜核不育系9012A不同于显性上位互作核不育,是一种新的基因互作核不育类型,其不育性受2对隐性重叠不育基因和1对隐性上位抑制基因互作控制,当隐性上位基因纯合时,对隐性不育基因起上位抑制作用。(2)在甘蓝型油菜中存在至少2套独立的不同隐性重叠不育基因,隐性上位基因与不同的隐性重叠不育基因之间的互作是非专一性的,这对于现有双基因隐性核不育杂种优势利用具有重要意义。 隐性上位互作核不育完全保持系的分离解决了传统隐性核不育杂种优势利用难点,为隐性核不育杂种优势利用开辟了新途径。这类核不育不仅在杂优育种实践中具有较高的开发利用价值,同时也是研究植物细胞核雄性不育机理的理想材料。 The results showed that: (1) The male sterile line 9012A of Brassica napus is different from the dominant male sterility, which is a new type of gene-interacting nuclear sterility. The sterility of male sterile line 9012A is affected by two pairs of recessive overlap The male sterile gene and one pair of recessive upper inhibitory genes interact with each other. When the recessive epistasis is homozygous, the recessive male sterility gene is upregulated. (2) There are at least two independent sets of recessive overlapping sterile genes in Brassica napus. The interaction between recessive epistatic genes and different recessive overlapping sterile genes is nonspecific, Binary recessive genic recessive heterosis utilization is of great significance. The separation of the recessive upper maintainer nuclear maintainers completely solved the difficulties in the utilization of heterotic recessive heterotrophic heterosis and opened a new way for heterosis utilization of recessive nuclear sterility. This kind of nuclear infertility not only has high value of exploitation and utilization in the practice of hybrid breeding, but also is an ideal material for studying plant male sterile mechanism of plant nucleus.