AIM:To study how intestinal lymph after trauma-inducedshock(TIS)interferes with expression of neutrophil adhesionfactors(CD11b and CD18)and causes lung injury.METHODS:Thirty-two adult healbhy Sprague-Dawley rats wererandomly divided into four experimental groups.Groups 1and 2 included rats with TIS caused by hitting the mid-upper part of both side femoral bones with a 2 500 kg raw-iron,and with or without ligation of mesenteric lymph duct.Groups 3 and 4 included rats with sham-TIS and with orwithout ligation of mesenteric lymph duct.Expression ofneutrophil CD18 and CD11b in at 1 and 3 h after a 90-minTIS/sham-TIS was evaluated.These rats were killed at 3 hafter TIS/sham-TIS,and lungs were taken immediately.The main lung injury indexes(the MPO activity and lung injuryscore)were measured.RESULTS:The expressions of CD18 and CD11b at 1 and 3 hafter a 90-min TIS and the main lung injury indexes weresignificantly increased compared with those in the sham-TIS groups(P<0.05).Moreover,at 1 and 3 h after TIS,theexpressions of CD18(32.12±1.25 and 33.46±0.98)andCD11b(29.56±1.35 and 30.56±1.85)were significantlydecreased in rats with ligation of mesenteric lymph duct,compared with those(52.3±1.12 and 50.21±1.25,and42.24±1.24 and 42.81±1.12,respectively)in those withoutthe ligation(all P<0.05).The main lung injury indexesin rats with TIS with ligation of mesenteric lymph duct(0.96±0.12 and 6.54±0.35)were also significantly decreased,compared with those(1.56±0.21 and 9.56±0.23)in ratswith TIS without the ligation(both P<0.05).However,therewas no significant difference in expressions of CD18 andCD11b and the main lung injury indexes between the twosham-TIS groups.CONCLUSION:Previous ligation of mesenteric lymph ductsprevents or alleviates the up-regulated expression of PMNCD18 and CD11b and the lung injury induced by TIS.Ourfindings also indicate that neutrophil adhesion moleculeactivation and lung injury during TIS appear to be caused bysome factors that are released or produced by post-ischemicintestine through the mesenteric lymph pathway.Chen ZB,Zheng SS,Yuan G,Ding CY,Zhang Y,Zhao XH,Ni LM.Effects of intestinal lymph on expression of neutrophil adhesion factors and lung injury after trauma-induced shock.World JGastroenterol 2004;10(21):3221-3224http://www.wjgnet.com/1007-9327/10/3221.asp AIM: To study how intestinal lymphosis after trauma-induced shock (TIS) interferes with expression of neutrophil adhesion factors (CD11b and CD18) and causes lung injury. METHODS: Thirty-two adult healbhy Sprague-Dawley rats were randomly divided into four experimental groups. Group 1 2 included rats with TIS caused by hitting the mid-upper part of both side femoral bones with a 2 500 kg raw-iron, and with or without ligation of mesenteric lymph duct. Groups 3 and 4 included rats with sham-TIS and with orwithout ligation of mesenteric lymph duct. Expression of neutrophil CD18 and CD11b in at 1 and 3 h after a 90-minTIS / sham-TIS was taken. These rats were killed at 3 hafter TIS / sham-TIS, and lungs were taken immediately.The main lung injury indexes (the MPO activity and lung injuryscore) were measured .RESULTS: The expressions of CD18 and CD11b at 1 and 3 hafter a 90-min TIS and the main lung injury indexes weresignificantly increased compared with those in the sham-TIS groups ( P <0.05) .Moreover, at 1 and 3 h after TIS, theexpressions of CD18 (32.12 ± 1.25 and 33.46 ± 0.98) andCD11b (29.56 ± 1.35 and 30.56 ± 1.85) were significantly decreased in rats with ligation of mesenteric lymph duct, compared with those (52.3 ± 1.12 and 50.21 ± 1.25, and42.24 ± 1.24 and 42.81 ± 1.12, respectively) in those without the ligation (all P <0.05). The main lung injury indexes in rats with TIS with ligation of mesenteric lymph duct (0.96 ± 0.12 and 6.54 ± 0.35) were also significantly decreased , compared with those (1.56 ± 0.21 and 9.56 ± 0.23) in rats with TIS without the ligation (both P <0.05) .Where, there was no significant difference in expressions of CD18 and CD11b and the main lung injury indexes between the twosham-TIS groups. CONCLUSION: Previous ligation of mesenteric lymph ductsprevents or alleviates the up-regulated expression of PMNCD18 and CD11b and the lung injury induced by TIS. Ourfindings also indicate that neutrophil adhesion moleculeactivation and lung injury during TIS appear to be caused bysome factors that are released o r producedby post-ischemicintestine through the mesenteric lymph pathway. Chen ZB, Zheng SS, Yuan G, Ding CY, Zhang Y, Zhao XH, Ni LM. Effects of intestinal lymph on expression of neutrophil adhesion factors and lung injury after trauma-induced shock. World JGastroenterol 2004; 10 (21): 3221-3224http: //www.wjgnet.com/1007-9327/10/3221.asp