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金黄色葡萄球菌核酸酶类似物(SNaseR)的酸变性态(U_A)在较高NaCl浓度下可转变为另一种稳定的酸变性态(A态),即U_A→A.CD谱测定结果表明A态结构中具有大量二级结构.体积排除色谱测定表明A态具有接近于天然态的堆积密度.A态可与疏水性荧光探针ANS结合.A态的这些特征表明其结构类似于熔球态.NaCl和HCl诱寻的U_A→A构象转变曲线相互重合,表明U_A→A转变是由C1~-与A态酶分子结合所引起的.C1~-结合到A态酶分子上稳定了该酶的α螺旋结构.盐酸胍可使A态发生变性.
The acid-labile state (U_A) of the S. aureus nuclease analog (SNaseR) transforms to another stable acid-denaturating state (state A) at higher NaCl concentrations, ie U_A → A. The results of the CD spectrometry A-state structure with a large number of secondary structure. Volume exclusion chromatographic determination showed that state A has a bulk density close to the natural state .A state can be combined with the hydrophobic fluorescent probe ANS .A state of these features indicate that the structure is similar to the melt ball State.NaCl and HCl-induced UA → A conformational transition coincide with each other, indicating that the UA → A transition is caused by the binding of C1 ~ to the A-state enzyme molecule.C1 ~ - binding to the A-state enzyme molecule stabilizes the The α-helical structure of the enzyme. Guanidine hydrochloride can denature the A-state.