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目的 观察阿魏酸钠 (sodiumferulate ,SF)对离体肝癌细胞生长的抑制作用。方法 将人EBL 740 4肝癌细胞以 1× 10 8/l浓度接种于 2 5cm2 细胞培养瓶中 ,接近单层时 ,分为SF组和对照组。SF组用含SF(终浓度为 1mg/l)和小牛血清 (5 0ml/l)的 16 40培养液培养细胞 ,对照组用含小牛血清 (5 0ml/l)的 16 40培养液培养细胞 ,48h后 ,收集细胞 ,碘化丙啶 (propidiumiodide ,PI)染色后 ,用流式细胞仪检测每一细胞周期的DNA含量 ,并根据DNA的含量分类细胞。 结果 (1)SF组细胞凋亡为 10 .5 % ,对照组为 5 .9% ,凋亡被上调。 (2 )SF组DNA百分比在S期明显降低 (SF组为 33 .12 % ,对照组为 47.83 % ) ,在G2 /M期明显增加 (SF组为 2 7.87% ,对照组为 15 .0 4% )。结论 SF诱导了人EBL 740 4肝癌细胞凋亡 ,抑制了人EBL 740 4肝癌细胞的增殖 ,SF能是一种有效抑制肝癌生长的中药。
Objective To observe the inhibitory effect of sodium ferulate (SF) on the growth of isolated hepatocellular carcinoma cells. METHODS: Human EBL7404 hepatoma cells were seeded into 25 cm2 culture flasks at a concentration of 1 x 10 8 /l. When they were close to monolayers, they were divided into SF group and control group. In the SF group, the cells were cultured in 1640 medium containing SF (final concentration of 1 mg/l) and calf serum (50 ml/l), and the control group was cultured in 1640 medium containing calf serum (50 ml/l). After 48 hours, the cells were collected and stained with propidiopyridine (PI). The DNA content of each cell cycle was determined by flow cytometry and the cells were sorted according to the DNA content. Results (1) The apoptosis of SF group was 10.5%, and that of control group was 5.9%. Apoptosis was up-regulated. (2) The percentage of DNA in SF group was significantly decreased in S phase (33.12% in SF group and 47.83% in control group), and it was significantly increased in G2/M phase (27.87% in SF group and 15.04 in control group). %). Conclusion SF induces apoptosis of human EBL 740 4 liver cancer cells and inhibits the proliferation of human EBL 740 4 liver cancer cells. SF can be a traditional Chinese medicine that can effectively inhibit the growth of liver cancer.