分泌型免疫球蛋白A(sIgA)抗血清是制备sIgA放免药盒的关键原材料,后者常用于判断机体的病毒、细菌感染或免疫功能。sIgA抗血清中含有人IgG抗体,必须去除以避免其对sIgA测定的干扰。原生产工艺所需的毒性试剂现今不易获得,本工作采用磁粒法免疫吸附处理sIgA抗血清,制备可用于放免药盒的sIgA抗体。用33%饱和硫酸铵两次沉淀、再经透析和上柱纯化,从人血清 Secreted immunoglobulin A (sIgA) antiserum is the key raw material for preparing sIgA radiopharmaceutical kit, which is often used to determine the body’s virus, bacterial infection or immune function. The sIgA antiserum contains human IgG antibody and must be removed to avoid interference with the sIgA assay. Toxicity reagents needed in the original production process are not readily available at present. In this work, the sIgA antiserum is treated by magnetic particle immunosorption to prepare the sIgA antibody that can be used for the radiopharmaceutical kit. Precipitate twice with 33% saturated ammonium sulfate and then dialyze and purify on column from human serum