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目的:采用蛋白质抗体芯片技术观察金黄扶正散对免疫抑制小鼠细胞因子的影响。方法:皮下注射环磷酰胺建立免疫抑制小鼠模型,金黄扶正散低、中、高剂量组分别连续给小鼠灌胃10 d,末次用药后小鼠禁食12 h,各组小鼠尾静脉取血1 mL,离心,取血清备用,采用细胞因子抗体芯片进行细胞因子定量检测。结果:与对照组比较,环磷酰胺组的2种细胞因子IFN-γ和RANTES呈显著性降低(P<0.05),金黄扶正散低、中、高剂量组给药后IFN-γ有显著性提升,高剂量组的RANTES呈显著性升高(P<0.05);与对照组比较,环磷酰胺组的5种细胞因子IL-5,IL-6,IL-9,IL-13和MCP-1呈显著性升高(P<0.01,P<0.05),给药组给药后有不同程度降低(P<0.01,P<0.05);13种细胞因子GM-CSF,IL-1α,IL-1β,IL-2,IL-3,IL-4,IL-10,IL-12,IL-17,M-CSF,TNF-α,KC和VEGF无明显变化。结论:金黄扶正散对免疫抑制小鼠20种细胞因子有不同程度的影响,这可能与调节免疫抑制小鼠Th1/Th2亚群有关。
OBJECTIVE: To observe the effects of Golden Harvest on the cytokines in immunosuppressed mice by protein antibody microarray. Methods: The immunosuppressive mice model was established by subcutaneous injection of cyclophosphamide. The mice in the low, medium and high dose groups of Golden Fuzheng San continuous administration were given gavage for 10 days, and the mice were fasted for 12 hours after the last administration. The tail vein Take 1 mL of blood, centrifuge, take serum reserve, using cytokine antibody chip for cytokine quantitative detection. Results: Compared with the control group, the levels of IFN-γ and RANTES in cyclophosphamide group were significantly decreased (P <0.05), and the levels of IFN-γ in the low, middle and high doses of Jinzhuang Fuzheng Sanjiao group were significantly decreased (P <0.05). Compared with the control group, the levels of IL-5, IL-6, IL-9, IL-13 and MCP- 1 were significantly increased (P <0.01, P <0.05), and the levels of GM-CSF, IL-1α and IL- 1β, IL-2, IL-3, IL-4, IL-10, IL-12, IL-17, M-CSF, TNF-α, KC and VEGF. Conclusion: Golden Fuzheng San has different effects on 20 cytokines in immunosuppressed mice, which may be related to the regulation of Th1 / Th2 subsets in immunosuppressed mice.