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目的:探索不同浓度氨基酸标准溶液的最佳衍生试剂用量。方法:通过取20μl衍生试剂所能衍生的氨基酸标准液的最高浓度样品,依次减少衍生试剂用量,根据数理统计学分析,比较各组峰面积与标准20μl所得峰面积有无显著性差异,探索各浓度范围的氨基酸标准溶液所需的最佳衍生试剂用量。结果:在浓度为0.75μmol/ml的氨基酸标准溶液中,加入17μl衍生试剂所得峰面积与标准20μl相比,无显著性差异;在浓度为0.5μmol/ml的氨基酸标准溶液中,加入15μl衍生试剂所得峰面积与标准20μl相比,无显著性差异。结论:当氨基酸浓度≤0.5μmol/ml时,衍生试剂用量为15μl即可充分衍生;对于浓度≤0.75μmol/ml的氨基酸标准溶液,17μl衍生试剂为最佳用量。
Objective: To explore the best derivatization reagent of amino acid standard solutions with different concentrations. Methods: By taking the highest concentration of amino acid standard solution that can derive from 20μl derivatization reagent, the amount of derivatization reagent was decreased in turn. According to the mathematical statistics analysis, whether there was any significant difference between the peak area of each group and the standard 20μl, Concentration of the amino acid standard solution required for the best amount of derivative reagent. Results: There was no significant difference in peak area of 17μl derivatization reagent when compared with the standard 20μl in the amino acid standard solution with the concentration of 0.75μmol / ml; in the amino acid standard solution with the concentration of 0.5μmol / ml, 15μl derivatization reagent The peak area obtained was not significantly different from the standard 20 μl. Conclusion: When the concentration of amino acids is less than or equal to 0.5μmol / ml, the derivatization reagent can be fully derivatized with 15μl. For the amino acid standard solution with concentration ≤ 0.75μmol / ml, 17μl derivative reagent is the best dosage.