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目的研究甲氨蝶呤(MTX)对类风湿关节炎(RA)滑膜细胞增生及细胞周期的影响,揭示MTX治疗RA的机制。方法应用MTS/PMS比色法和流式细胞术分别测定MTX处理和未处理RA患者滑膜细胞的细胞增生水平和细胞周期。结果加入不同浓度MTX(0.2~2.0 μmol/L),RA患者滑膜细胞接种后第4天起增生水平均显著低于未处理的RA患者滑膜细胞(P<0.05);细胞周期分析显示加入不同浓度MTX(0.2~2.0 μmol/L)后第5、8天,RA患者滑膜细胞停留在G1期的比例要比未处理的RA患者滑膜细胞明显增加(P< 0.05),而S、G2期的细胞比例比未处理的RA患者滑膜细胞则明显减少(P<0.05);MTX能以剂量依赖性明显抑制滑膜细胞的增生水平。结论MTX在治疗RA时,主要起到对RA患者滑膜细胞的增生及增生相关病理过程的抑制。
Objective To investigate the effect of methotrexate (MTX) on the proliferation and cell cycle of rheumatoid arthritis (RA) synovial cells and to reveal the mechanism of MTX in the treatment of RA. Methods MTS / PMS colorimetric assay and flow cytometry were used to determine the cell proliferation and cell cycle of synoviocytes in MTX-treated and untreated RA patients. Results The proliferation of synovial cells from 4 days after inoculation of RA patients was significantly lower than that of untreated RA patients (P <0.05) by adding different concentrations of MTX (0.2-2.0 μmol / L). Cell cycle analysis showed that the addition of The synoviocytes in RA patients stayed in the G1 phase at 5 and 8 days after MTX (0.2-2.0 μmol / L), but the synoviocytes in RA patients were significantly increased than those in untreated RA patients (P <0.05) The proportion of cells in G2 phase was significantly lower than that in untreated RA patients (P <0.05). MTX significantly inhibited the proliferation of synovial cells in a dose-dependent manner. Conclusions MTX plays an important role in inhibiting the proliferation and proliferation of synovial cells in RA patients.