trypsin相关论文
为了进一步研究胰蛋白酶的保鲜机制,探讨火龙果中胰蛋白酶和抗氧化酶的协同机制,根据胰蛋白酶调控火龙果的RNA-seq数据筛选活性氧(......
The KNRL nuclear receptor controls hydrolase-mediated vitellin breakdown during embryogenesis in the
Vitellin (Vn) homeostasis is central to the fecundity of oviparous insects.Most studies have focused on the synthesis an......
It is challenging to develop an in vitro catalytic system to conduct natural surface-confined enzymatic reactions in a s......
The full length cDNA of trypsin (TRY) gene was cloned from hepatopancreas of Macrobrachium rosenbergii with reverse tran......
Study on the preparation of peptides from Scapharca broughtonii using immobilized trypsin by chitosa
...
Integrated platform of capillary isoelectric focusing,trypsin immobilized enzyme microreactor and na
In proteomic study, top-down and bottom-up approaches have been widely adopted.However, each approach has its own weakne......
Several recent reports [1-2] have highlighted the speed and convenience of microwave-assisted enzymatic digestion.Howeve......
In proteome studies, proteolytical digestion is a necessary step for the identification of proteins by mass spectrometry......
...
It is challenging to develop an in w\fro catalytic system to conduct natural surface-confined enzymatic reactions in a ......
Recent studies suggest that PRL-3 is involved in the metastasis of colorectal cancer, but the mechanism concerning that ......
AIM: To identify gene mutations in PRSS1 and SPINK1 in individuals with early onset idiopathic chronic or recurrent acut......
采用ＳＴ１－Ｓｅｐｈａｒｏｓｅ ４Ｂ亲和层析的方法，从鼠新鲜胰脏中分离得到纯的胰蛋白酶。大鼠胰蛋白酶的比活为２４６１５ＢＡＥＥＵ／ｍｇ蛋白，总活性回收率４７％；小鼠胰蛋白酶的比活为３２７６８ＢＡＥＥＵ／ｍｇ蛋白，总活......
本文基于酶的纯化鉴定、动力学分析、序列比对和结构预测,对南极磷虾胰蛋白酶的结构及适冷性进行研究,通过与其他几种适冷、中温胰......
卵巢癌是一种致死性妇科恶性肿瘤,大部病人在晚期才诊断,而无有效疗法。一种新的肿瘤标记CA125用于诊断卵巢癌,80%的卵巢癌患者血......
Human blood coagulation factor XI (FXI) is a key enzyme in the amplification phase of blood coagulation cascade,and is r......
Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-a......
A novel method for identification and relative quantification of N-terminal peptides using metal-ele
By means of the reaction between a DOTA-NHS-ester bifunctional reagent and N-terminal peptides of proteins,and then chel......
Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm,Spodoptera exigu
Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet ar......
Molecular dynamics simulation on BPTI (biovine pancreatic trypsin inhibitor) aqueous solution has been performed for the......
As a trypsin-like serine protease, Urokinase-type plasminogen activator (uPA) plays a key role in a range of biological ......
Relationship between Insuline-like Growth Factor-I and Progesterone Secretion of Cultured Human Trop
Objective To investigate the effect of insuline-like growth factor-I (IGF-I) on proges- terone genesis and regulation. M......
本文分别测定了重组的与天然的牛胰蛋白酶和羧肽酶B对前胰岛素融合蛋白的裂解活性。水解产物采用HPLC进行分析,发现重组酶具有更强......
AIM To investigate whether gut maturation could be induced precociously in an athymic T-cell deficient neonatal rat mode......
为了揭示饲粮中油菜籽不同添加量对牦牛瘤胃和十二指肠食糜消化酶活性的影响及各酶活性间的关系,试验将18头年龄、性别和体况一致......
Objective: To investigate the differences of primary culture, purification and biological characteristics between endoth......
AIM: To investigate how the saturated and unsaturated fatty acid composition influences the susceptibility of developing......
The amino acid sequence of a double-headed trypsin inhibitor from the seeds of Momordica charantia L
A double-headed trypsin inhibitor(MCI-1)was isolated and purified from the seedsof Momordica charantia Linn.Cucurbitace......
Preliminary identification of C-terminus of trichosanthin by chemical and enzymic methods,such as hydrazinolysis,thiohy......
Polyvinyl alcohol carriers bearing active amide and active ester groups were prepared by acetaliza-tion of granular mac......
Assessment of Rhizospheric Microorganisms of Transgenic Populus tomentosa with Cowpea Trypsin Inhibi
To have a preliminary insight into biosafety of genetically transformed hybrid triploid poplars (Populus tomentosa × P.......
Objective To explore the role of coxsackievirus and adenovirus receptor(CAR)in cardiotoxicity infected by coxsackievirus......
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been combined with immobilized enzyme micr......
Formaldehyde,a well-known environmental toxic hazard,has been found to produce endogenously via semicarbazide-sensitive ......
Activity Of Bacterial Proteolytic Enzymes on Antinutritional Factors in Soybeans and the Effect on G
A significant reduction of trypsin inhibitory activity by selected bacterial proteolytic enzymes was demon-rnstrated in ......
PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H_(11) AGAINST G
The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H_(11) against g......
AIM To determine the activities ofpolysaccharide extracts from Flammulina velutipes (Curt. ex Fr. ) Sing (FV), Lentinuse......
EFFECT ON BIOLOGICAL BEHAVIOR OF CHEMOTHERAPY-RESISTANT TUMOR CELLS BY HUMAN WILD-TYPE p53,GM-CSF AN
Objective: To explore the effect on biological behavior of chemotherapy-resistant tumor cells by human wild-type p53, GM......
托拉塞米(TOR)属于吡啶磺酰脲类袢利尿剂,被广泛有效地用于高血压,心脏衰竭,慢性肾功能衰竭和肝脏疾病的治疗。TOR在治疗过程中易......
The Effects of Dense/Nanometer Hydroxyapatite on Proliferation and Osteogenetic Differentiation of P
The objective of this study is to investigate possible effects of nanometer powder of hydroxyapatite on proliferation of......
Fe_3O_4 magnetic nanoparticles were prepared by co-precipitation of Fe2+ and Fe3+ in an ammonia solution,and its size wa......
隐孢子虫卵囊的无菌化及体外脱囊是进行虫体体外细胞培养不可缺少的环节，本试验通过使用不同浓度次氯酸钠提前处理卵囊，在不同脱囊液......
Kazal-type serine protease inhibitor is one of the most important and widely distributed protease inhibitor families.Pan......
