本文以稻属十二个野生种及两个远缘杂种的茎节为材料,外植体在 N_6添加 NAA0.5mg/l,2,4-D 2mg/l 和3%蔗糖的培养基上诱导愈伤组织,继尔转入 MS 培养基促成体细胞胚分化。结果表明:所有接种材料均能长出愈伤组织,出愈率范围为7.4—48.0%,平均出愈率为20.7%。除重颖野生稻、紧穗野生稻及印度野生稻未分化植株外,其余野生种均产生试管植株,绿苗再生率为0—50% In this paper, the stem segments of twelve wild species and two distant hybrids were used as explants. The explants were induced on N6 medium supplemented with NAA 0.5 mg / l, 2,4-D 2 mg / l and 3% sucrose Callus, transferred to MS medium to promote somatic embryo differentiation. The results showed that all the inoculated materials could grow callus, the curative rate ranged from 7.4 to 48.0% and the average curative rate was 20.7%. In addition to re-wild rice, tight-grafted wild rice and Indica wild rice undifferentiated plants, the remaining wild species produce test-tube plants, green seedling regeneration rate of 0-50%