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目的研究 CD44v3,v6蛋白在结直肠癌组织中的表达及其预后意义.方法应用 Envision~(TM)免疫组化法,回顾性分析121例结直肠癌石蜡组织 CD44v3,v6的表达.患者中位随访时间为67.77mo.结果 CD44v3,v6在结直肠癌中的阳性率分别为60.3%和57.9%.CD44v3的阳性表达与患者肿瘤部位,淋巴结转移状况、远处转移与否、临床分期密切相关(P<0.05,Spearman 等级相关检验).CD44v6的阳性表达与患者性别、淋巴结转移状况、远处转移与否、临床分期密切相关(P<0.05,Spearman 等级相关检验).Kaplan-Meier 生存曲线,Log-rank 检验单因素生存分析结果显示,CD44v3阴性、阳性患者的五年生存率分别为81.25%,60.27%,两者之间存在显著性差异(P=0.035);CD44v6阴性、阳性患者的5a 生存率分别为80.39%,60.00%,两者之间亦存在显著性差异(P=0.0299).Cox 模型多因素生存分析结果显示,CD44v3表达是影响结直肠癌预后的独立因素.结论 CD44v3,v6蛋白与结直肠癌转移、预后相关,CD44v3是影响结直肠癌预后的独立因素.
Objective To investigate the expression of CD44v3 and v6 protein in colorectal cancer and its prognostic significance. Methods Envision~(TM) immunohistochemistry was used to analyze the expression of CD44v3 and v6 in paraffin tissue from 121 cases of colorectal cancer. The follow-up time was 67.77mo. Results The positive rates of CD44v3 and v6 in colorectal cancer were 60.3% and 57.9%, respectively. The positive expression of CD44v3 was closely related to tumor site, lymph node metastasis status, distant metastasis or not, clinical stage ( P <0.05, Spearman rank correlation test.) The positive expression of CD44v6 was closely related to gender, lymph node metastasis, distant metastasis or not, clinical stage (P <0.05, Spearman rank correlation test). Kaplan-Meier survival curve, Log -rank test univariate survival analysis showed that the 5-year survival rate of CD44v3-negative and positive patients was 81.25% and 60.27% respectively. There was a significant difference between them (P=0.035); 5-year survival of CD44v6-negative and positive patients. The rates were 80.39% and 60.00%, respectively, and there was also a significant difference between them (P=0.0299). The Cox model multivariate survival analysis results showed that CD44v3 expression was an independent factor influencing the prognosis of colorectal cancer. Conclusion CD44v3, v6 White and colorectal cancer metastasis, prognosis, of CD44v3 independent prognostic factors in colorectal cancer.