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目的 观察补肾醒脑解毒中药复方对 Ｈ Ｓ Ｖ－ １感染神经元的保护作用。方法 用 Ｈ Ｓ Ｖ－ １感染原代培养大鼠海马神经元造成急性感染与潜伏感染，然后检测中药煎液对神经元细胞病变及子代病毒滴度的影响。结果 培养神经元感染０１ Ｍ Ｏ Ｉ Ｈ Ｓ Ｖ－ １后２４ ｈ 出现病变，６ｄ 后７０％ 的细胞溶解死亡。补肾醒脑解毒方对低感染量病毒（００５ Ｍ Ｏ Ｉ）所致细胞病变有较强的抑制作用，并能降低子代病毒滴度。对潜伏感染神经元能维持其正常形态１０周以上。结论 补肾醒脑解毒方能维持潜伏感染神经元正常形态，并能减轻 Ｈ Ｓ Ｖ－ １所致神经元急性溶细胞病变。
Objective To observe the protective effect of Bushen Xingnao Detoxification Chinese Herbal Compound Compound on HSV-1-infected neurons. Methods The primary cultured rat hippocampal neurons were infected with H S V-1 to cause acute infection and latent infection. The effects of Chinese herb decoction on neuronal cell lesions and offspring virus titers were then measured. Results The lesions appeared 24 h after cultured neurons infected with O 1 M O I H S V-1, and 70% of the cells died after 6 days. Bu Shen Xing Nao Jie Du Fang has a strong inhibitory effect on the low-infection virus (005 M O I)-induced cytopathic effect, and can reduce the offspring virus titers. The latently infected neurons can maintain their normal morphology for more than 10 weeks. Conclusion Bushen Xingnao Jiedu can maintain the normal morphology of latently infected neurons, and can reduce the acute cytolysis of neurons caused by H S V-1.